Fig 1.
Microscopic observations of powdery mildew infection in C. moschata genotypes.
Non-inoculated leaves of “112–2” (A), 24 h of PM-inoculated leaves of “112–2” (B), 48 h of PM-inoculated leaves of “112–2” (C), Non-inoculated leaves of “JJJD” (D), 24 h of PM -inoculated leaves of “JJJD” (E), and 48 h of PM-inoculated leaves of “JJJD” (F). The arrow indicates the growth of PM pathogen, h, hyphal; c, conidium; bt, bud tube.
Fig 2.
Analysis of unigene expression difference in pumpkin.
The x-axis represents the unigene expression fold changes between any two libraries; the y-axis represents the statistically significant analysis of unigene expression differences. The smaller the adjusted call (padj), the greater and more significant the -log10 (padj). The scattered points represent each gene. No significant difference is indicated by blue color, whereas significant up-regulation and down-regulation are indicated by red and green colors, respectively.
Fig 3.
A, DEGs at 24 h after PM inoculation compared with untreated control plants; B, DEGs at 48 h after PM inoculation compared with untreated control plants. The y-axis indicates the number of genes in a subcategory, and the x-axis indicates the different subcategories.
Fig 4.
Analysis of the mRNA expression of 8 genes using RT-qPCR in PM-resistant and PM-susceptible plants.
Total RNA was extracted from pumpkin leaves that were sprayed with a spore suspension or water. The pumpkin β-actin gene was used as an internal reference gene. The expression levels of the genes of plants sprayed with water only at each time point were used as controls. The relative gene expression in A, F and G (Y-axis) was transformed to a log10 scale. The values are the means ± SEs of three biological replicates.*indicates significant differences (P<0.05).
Fig 5.
Analysis of the mRNA expression of other 8 genes using RT-qPCR in PM-resistant and PM-susceptible plants.
Total RNA was extracted from pumpkin leaves that were sprayed with a spore suspension or water. The pumpkin β-actin gene was used as an internal reference gene. The expression levels of the genes of plants sprayed with water only at each time point were used as controls. The relative gene expression in A, B, C, D, E and G (Y-axis) was transformed to a log10 scale. The values are the means ± SEs of three biological replicates.*indicates significant differences (P<0.05).