Fig 1.
Mapping of ArfGAP1 localization in the adult mouse brain.
A. Coronal plane. B. Sagittal plane. C. Double labeling of ArfGAP1(green) and Nissl st. (stain) (red) of cortical layers (L). IsoCtx–isocortex, RSP–retrosplenial cortex, VIS–visual cortex, PTLp–posterior parietal association areas, MO–motor cortex, SS–somatosensory cortex, AUD–auditory cortex, TEa–temporal association areas, ECT–ectorhinal cortex, PERI–perirhinal cortex, ENTI–entorhinal cortex, FRP–frontal pole, ORB–orbital area, OLF–olfactory cortex, OB–olfactory bulb, STR–striatum, AMG–amygdala, FT–fiber tracts, TH–thalamus, HY–hypothalamus, HIP–hippocampus, VL–lateral ventricle, PAL–pallidum, MB–midbrain, P–ponce, MY–medulla, Cer–cerebellum, arb–arbor vitae, CP–cerebral peduncle, SC–spinal cord. The scale bars represent 500 μm (A,B) and 200 μm (C).
Fig 2.
ArfGAP1 immunoreactivity in the hippocampus.
A. Coronal plane. B. schematic view of the mossy fibers projections. C. Sagittal plane. SP-MF–suprapyramidal bundle and IIP-MF–intra- and infrapyramidal bundles of the mossy fibers projections. C. Sagittal plane. poDG–polymorph layer (hilus) of the DG, grDG–granular cell layer of the DG, sr–stratum radiatum, slu–stratum lucidum, so–sorstratum oriens. CA1, CA2 and CA3 –Cornu Ammonis of the hippocampus. The scale bars represent 200 μm.
Fig 3.
Western blot detection of total ArfGAP1 and ArfGAP1B in adult mouse brain regions.
A. Data were normalized to GAPDH protein levels for each region. DG–dentate gyrus, Cer–cerebellum, OB–olfactory bulb, IsoCtx–isocortex, TH–thalamus. Data expressed as the mean ± SEM, n = 3. B. Linear regression analysis of the pairs of data obtained using the two antibodies from each tissue in each of the 3 experiments.
Fig 4.
ArfGAP1 protein localization in mouse dentate gyrus and cerebelum.
A, B. coronal view of the DG, poDG–polymorph layer (hilus) of the DG, grDG–granular cell layer of the DG, sr–stratum radiatum, slu–stratum lucidum, so–sorstratum oriens. CA1, CA2 and CA3 –Cornu Ammonis of the hippocampus. C, D. Coronal view of the cerebellum, ANcr1 and ANcr2 –ansiform cruciform lobule 1 and 2, arb–arbor vitae; ArfGAP1 (green), DAPI stain (blue). B,D–control experiments with the omission of primary antibody and staining only with secondary antibody. Magnification 10X. The scale bars represent 210 μm.
Fig 5.
ArfGAP1 is localized in the mossy fibers of the mouse DG.
A, B. Double labeling of ArfGAP1(green) and ZnT3 (red). Yellow staining indicates colocalization of ArfGAP1 and ZnT3. C, D. Double labeling of ArfGAP1(green) and GFAP (red). F, E. Omission of the primary antibody and staining only with secondary antibody. Abbreviations: CA1, CA2 and CA3 –Cornu Ammonis of the hippocampus. Coronal DG (dentate gyrus) view of ventral (A, C, F) and dorsal (B, D, E) hippocampus. TH–thalamus. mf–mossy fibers. Magnification 10X.The scale bars represent 210 μm.