Fig 1.
Symptoms of citrus greasy spot.
A, leaves of ‘Valencia’ sweet orange in Churuquita, Panama B, leaves of ‘Ortanique’ tangor in Alzira, Spain C, chlorotic lesions on the adaxial leaf surface of ‘Valencia’ sweet orange plants inoculated with isolates of the Zasmidium citri-griseum complex from Panama D, necrotic pustules on the abaxial leaf surface E and F, absence of greasy spot symptoms in control plants.
Table 1.
Details of the isolates and sequences of Mycosphaerellaceae included in the study.
Fig 2.
A Bayesian 50% majority rule consensus tree based on a combined ITS and EF-1α alignment, containing Mycosphaerellaceae isolates from citrus.
Bayesian posterior probabilities support values for the respective nodes are displayed in the tree. The tree was rooted to Pseudocercospora angolensis (CBS112933).
Fig 3.
Colony morphology of Mycosphaerellaceae isolates: Zasmidium citri-griseum (4NTV1), Amycosphaerella africana (MC-140), reference isolate of Z. citri-griseum (CBS 122455), reference isolate of A. africana (CBS 680.95, CBS 110500, CBS 110843) on potato dextrose agar (PDA), oatmeal agar (OA), spezieller nährstoffarmer agar (SNA), and malt extract agar (MEA) incubated at 25°C in the dark for 30 days.
Fig 4.
Responses to temperature (T) of radial mycelial growth rate (Y) denoted by a normal distribution of mean μ and standard deviation σ.
A, Zasmidium citri-griseum complex isolates (n = 25). B, Amycosphaerella africana (n = 21).
Table 2.
Severity of citrus greasy spot on ‘Valencia’ sweet orange plants inoculated with isolates of the Zasmidium citri-griseum complex from Panama and percentage of reisolation.