Fig 1.
Phylogenetic analysis of GII norovirus based on the partial nucleotide sequences of the polymerase and capsid regions, using the MON431 (nt 4820–4839) and G2SKR (nt 5367–5389) primers.
(a) Phylogenetic tree of 231 bp within the polymerase region (3’-ORF1). (b) Phylogenetic tree of 277 bp within the capsid region (5’-ORF2). References strains of norovirus genotypes are named according to GenBank with their respectively accession numbers. Brazilian strains are marked with black filled diamonds. Recombinant strains of GII.P16-GII.4 are marked with green filled diamonds, and recombinant strains of GII.P16 grouping with non-GII.4 capsid genotypes are highlighted with unfilled green diamonds. The bootstrap values (2,000 replicates) are indicated in the phylogenetic tree, and values less than 70% are not represented. The bar at the bottom of the figure is proportional to the genetic distance.
Table 1.
Distribution of norovirus genotypes detected in samples collected between January 2015 and July 2016 (n = 37) of the southeastern Brazilian coast state, Espírito Santo.
Fig 2.
Alignment of sequences of VP1 protruding P domain derived from norovirus GII.4 strains.
Antigenic epitopes A (green), B (blue), C (orange), D (purple) and E (red) are indicated.