Table 1.
List of primer sequence and related information for each candidate reference gene.
Fig 1.
Expression stability and ranking of 9 reference genes under SMV treatment as determined by geNorm.
(A) Leaves of ZD32 at 15 min and 6 h post-inoculation with SMV and control. (B) Leaves of ZD29 at 15 min and 6 h post-inoculation with SMV and control. (C) Leaves of ZD32 and ZD29 at 15 min post-inoculation with SMV. (D) Apical buds of TL1 at VE, V1 and V3 stages. (E) Apical buds of TL2 at VE, V1 and V3 stages. (F) Apical buds of TL1 and TL2 at VE and V1 stages. (G) Shoots of PH under nitrogen stress. (H) Roots of PH under nitrogen stress. (I) Shoots and roots of PH under nitrogen stress. (J) Leaves of ZD32 inoculated with SMV, apical buds of TL1 at VE stage and leaves of PH under nitrogen stress.
Table 2.
Average standard devation (SD) of delta Ct of RT-qPCR.
Fig 2.
Gene expression pairwise variation (V) of the candidate reference genes calculated by geNorm.
The pairwise variation (Vn/n+1) was analyzed between the normalization factors NFn and NFn+1 by geNorm program to determine the optimal number of reference genes required for effective normalization of RT-qPCR data.
Table 3.
Recommended reference genes for RT-qPCR of soybean as predicted by geNorm and ΔCt algorithms.