Fig 1.
Scanning electron microscopy images of dehydrated cells of D. radiodurans deposited on aluminum plates and used in experimental set up of Tanpopo mission.
(A, B) Scanning electron microscopy images, showing upper surface and inner content of multilayers of dehydrated D. radiodurans cells deposited on aluminum plates. (C, D) Higher magnification images displaying upper surface of multilayers of dehydrated cells of D. radiodurans. (E, F) Magnified images of tetracocci and diplococci of D. radiodurans taken from the inner part of dehydrated multilayers. (A, C, E) control cells of D. radiodurans; (B, D, F) cells of D. radiodurans exposed to UVC-vacuum conditions.
Fig 2.
First two levels of gene ontology annotations of all proteins of D. radiodurans, which were identified in at least three out of four replicates in at least one condition.
Fig 3.
PCA score-plot of the z-scored label free quantification intensities.
A clear separation can be observed on the PC1 level, which explains 34.62% of the data’s variance, between the UVC/vacuum treated samples (red) and the control samples (green).
Fig 4.
Bar plot of KEGG categories (x-axis) with corresponding enrichment factors (y-axis).
Categories with a minimum enrichment factor of two for either UVC/vacuum (blue) treated or control (red) conditions are mapped. An enrichment factor of zero means that not a single protein in this category was upregulated in the displayed condition.
Fig 5.
Boxplot of genes encoding important damage response proteins in D. radiodurans under the conditions of UVC/vacuum exposure.
For every gene, the z-scored LFQ intensities are compared between the control and UVC/vacuum condition. The lower and the upper hinges correspond to the first and the third quartiles. The whiskers extend a maximum of 1.5 times the inter-quartile range. Outliers are indicated as dots. Proteins which are encoded by the mapped genes: Clp protease subunits (clpP and clpX), DNA damage response proteins (ddrB and ddrD), chaperone (dnaK), DNA gyrase subunit A (gyrA), radiation response metalloprotease (irrE), DNA polymerase (polA), DNA repair protein (pprA), recombinase (recA), single-stranded DNA-binding protein (ssb); catalase (katA), uncharacterized protein (DR_A0146), superoxide dismutase (sodA), phytoene dehydrogenase (DR_0861), Pyridoxal 5’-phosphate synthase (pdxS and pdxT), thioredoxin reductase (DR_1982), putative peroxidase (DR_A0145), peptide methionine sulfoxide reductase (msrA), tellurium resistance protein (DR_2220 and DR_2221).
Fig 6.
Heatmap of metabolites, which were considered different between cells of D. radiodurans exposed to UVC/vacuum and non-exposed control cells.
Eucledian distance was used for calculating the dendrogram. *Identification was based on database research and not on a reference substance.
Fig 7.
Main components of the TCA cycle in Deinococcus radiodurans connected to related pathways under the conditions of UVC/vacuum exposure.
Metabolites are shown as rectangles. The areas of the proteins, which are shown as circles, correspond to the fold change between cells of D. radiodurans exposed to UVC/vacuum conditions and control non-exposed cells. The color shows whether the average protein or metabolite level was more abundant in the UVC/vacuum exposed cells (blue), the control cells (red), none of both conditions (yellow) or not measured/not abundant enough (colorless).
Fig 8.
Molecular response of D. radiodurans experienced under UVC and vacuum conditions.
First two levels of molecular pathways are represented which are affected by UVC irradiation under vacuum conditions.