Fig 1.
Statistic of differentially expressd genes (DEGs) of different citrus cultivars in response to CLas.
CH, Citrus hystrix; CS, C. sinensis; M, Mock/healthy; HLB, Huanglongbing; CH-M-VS-CH-HLB, DEGs in HLB-infected C. hystrix compared with healthy control; CS-M-VS-CS-HLB, DEGs in HLB-infected C. sinensis compared with healthy control; CS-M-VS-CH-M, DEGs between healthy C. hystrix and healthy C. sinensis; CS-HLB-VS-CH-HLB, DEGs between HLB-infected C. hystrix and HLB-infected C. sinensis.
Fig 2.
Gene ontology classification of differentially expressed genes in different citrus hosts in response to ‘Candidatus Liberibacter asiaticus’.
BP, Biological Process; CC, Cell Component; MF, Molecular Function.
Fig 3.
Comparative PageMan display of perturbed pathways in CLas-affected C. hystrix and C. sinensis.
The log2 fold change of gene expression (mock-inoculated controls versus CLas-inoculated plants) was input into PageMan and subjected to a Wilcoxon test. Results were shown as a false-color heat-map-like display. Significantly up-regulated pathways are colored in red, while those colored in green are significantly down-regulated. Pathways without significant changes are white. Names of pathways are indicated on the right panel. CH, CH-M-VS-CH-HLB; CS, CS-M-VS-CS-HLB.
Fig 4.
Differentially expressed genes involved in starch metabolism (A) and photosynthesis (B).
Colored squares indicate up- or down-regulated genes with log2 fold change (FC) ≥ 1.00 or ≤ -1.00.
Fig 5.
MapMan analysis of differentially expressed genes in CLas-affected Citrus hystrix (A) and CLas-affected C. sinensis (B) involved in stress responses.
Red squares represent genes those were significantly up-regulated; green squares represent genes those were significantly down-regulated.
Fig 6.
RT-qPCR and RNA-seq profiles of 16 selected differentially expressed genes (DEGs).
A. DEGs between healthy and HLB-infected Citrus sinensis; B, DEGs between healthy and HLB-infected C. hystrix; C, DEGs between HLB-infected C. hystrix and HLB-infected C. sinensis. glgA, starch synthase; TPP, trehalose 6-phosphate phosphatase; LHCb1, light-harvesting complex II chlorophyll a/b binding protein 1; PAL, phenylalanine ammonia-lyase-like; 2OG-Fe(II), 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase; CESA, cellulose synthase A; XTH23, xyloglucan endotransglucosylase/hydrolase protein 23; PHT, phosphate transporter; ZIP2, zinc transporter 2; EREBP, ethylene-responsive transcription factor ERF017; SODC, superoxide dismutase [Cu-Zn]; LRR-1, LRR receptor-like serine/threonine-protein kinase; LRR-2, LRR-like serine/threonine-protein kinase BAM2; RGA3, disease resistance protein RGA3, NB-ARC class; EDS1 (enhanced disease susceptibility 1 protein); MIR, miraculin.