Table 1.
Detailed information for the EgWRKY genes identified in the genome of Elaeis guineensis.
Fig 1.
Distribution of EgWRKY genes across the chromosomes of Elaeis guineensis.
Vertical bars represent chromosomes of Elaeis guineensis. The chromosome number is indicated at the top of each chromosome. The coloured lines indicate different clusters based on multiple alignments between conserved WRKY domains from the 95 EgWRKY genes identified in this study. Dark red: cluster I; Green: cluster II; Blue: cluster III; Yellow: cluster IV; Red: cluster V; Black: cluster VI; Purple: cluster VII; Brown: cluster VIII; Bright green: cluster IX.
Fig 2.
Gene structures of the 95 EgWRKY genes identified in this study.
Exons are represented by coloured boxes and introns are represented by black lines.
Fig 3.
Duplicated genes pairs of EgWRKYs identified in this study.
The coloured bars represent chromosomes of Elaeis guineensis.
Fig 4.
Schematic representation of conserved motifs in 95 EgWRKY genes.
All motifs were identified by MEME with the full-length amino acid sequence of 95 EgWRKY in Elaeis guineensis. The size of each EgWRKY were displayed proportionally. WRKY and bZIP motif were indicated using different colour box.
Fig 5.
Phylogenetic tree obtained from the neighbour-joining analysis of conserved WRKY motifs from 95 EgWRKY genes.
EgWRKYs that contain only one WRKY domain are marked with brown circles; EgWRKYs that contain conserved WRKY and bZIP domains are marked with red circles; EgWRKYs that contain two conserved WRKY domains are marked with blue circles.
Fig 6.
Heat map of EgWRKY gene expression in different tissues of Elaeis guineensis.
Log 2RPKM values were used to construct the heat map with clustering.
Fig 7.
The expression patterns of duplicate genes in different tissues.
Fig 8.
Real-time quantitative PCR validation of EgWRKY gene expression under salt, drought and cold treatment.
One-way ANOVA was used to test significant difference of expression level between adjacent time periods. * indicated “p< 0.05” and ** indicated “p< 0.01”.