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Fig 1.

Maps of the Sierra de Guadarrama National Park.

(A) basic orography. (B) habitats that occupy greater surfaces [36]. The spatial location of the sampling points is indicated and numbered.

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Fig 2.

Installation of Subterranean Sampling Device (SSD).

(A) Scree slopes from shadowed areas of La Mujer Muerta (Segovia). (B) Scree slopes from Cancho del Río Peces (Segovia). (C) SSD before being buried. (D) SSDs of 1 m (left) and of 0.5 m (right) in the process of installation. (E) Placing of the pitfall trap inside the cylinder (F–I) SSD used in sampling.

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Table 1.

Location of the traps.

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Fig 3.

Habitus and color pattern of the Orchesella species studied.

(A) Orchesella mesovoides sp. nov., lateral view. (B) Orchesella mesovoides sp. nov., dorsal view. (C) Orchesella eolia, dorsal view. (D) Orchesella colluvialis sp. nov., lateral view. (E) Orchesella villosa, lateral view. (F) Orchesella cincta, lateral view (bar 0.5 mm).

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Fig 4.

Antennae of the Orchesella species studied.

(A–B) Orchesella mesovoides sp. nov. aberrant antenna, with a flagellus instead of the normal third and fourth segments and a normal antenna with some areas enlarged. (C–D) Orchesella colluvialis sp. nov. antenna. (E), Orchesella villosa. (F–G) Orchesella cincta (bar for antennae: 0.25 mm; bar for enlarged areas: 0.05 mm).

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Fig 5.

Some morphological characters of the Orchesella species studied.

(A) Trochanteral organ of Orchesella mesovoides sp. nov. (B) Trochanteral organ of Orchesella colluvialis sp. nov. (C) Trochanteral organ of Orchesella villosa. (D) Trochanteral organ of Orchesella cincta. (E) Tip of the leg III (claw and empodial appendage, anterior view) of Orchesella mesovoides sp. nov. (F) Tip of the dens and mucro of Orchesella mesovoides sp. nov. (G) Tenaculum of Orchesella mesovoides sp. nov. (H) Tip of the leg III of Orchesella colluvialis sp. nov. (I) Tip of the dens and mucro of Orchesella colluvialis sp. nov. (J) Maxilar outer lobe of Orchesella colluvialis sp. nov. (K), Tip of the leg III of Orchesella villosa (the arrow points to the absent tooth in the drawn claw). (L) Tip of the dens and mucro of Orchesella villosa. (M) Tip of the leg III of Orchesella cincta. (N) Tip of the dens and mucro of Orchesella cincta. (O–Q) Macrochaetae from tibiotarsus of leg III of the Orchesella colluvialis sp. nov.: long, special and short (normal size), respectively (bars: A–B: 0.1 mm; E–F, H–I, K–L, M–N: 0.5 mm; G: 0.04 mm; J, O–Q: 0.02 mm).

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Fig 6.

SEM photographs.

(A) Eye of the Orchesella mesovoides sp. nov. (bar 0.04 mm). (B) Eye of the Orchesella colluvialis sp. nov. (bar 0.04 mm). (C) Tip of the antennal segment II and the basal part of III of the Orchesella mesovoides sp. nov. (bar 0.09 mm). (D) Detail of the antennal segment III in an area with apparent segmentation of the Orchesella mesovoides sp. nov. (bar 0.006 mm). (E) S-microchaeta from the Abd III of the Orchesella colluvialis sp. nov. (bar 0.004 mm). (F) S-chaeta from the Abd II of the Orchesella mesovoides sp. nov. (bar 0.006 mm). (G) S-chaeta from the Abd IV of the Orchesella mesovoides sp. nov. (bar 0.02 mm).

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Fig 7.

Head chaetotaxy of the Orchesella species studied.

(A) Orchesella mesovoides sp. nov.; (B) Orchesella colluvialis sp. nov. (C) Orchesella villosa. (D) Orchesella cincta. (E) Eye details of the Orchesella mesovoides sp. nov. (F) Main chaetotaxy of Orchesella eolia (bar 0.1 mm except for E, 0.04 mm).

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Fig 8.

Dorsal macrochaetotaxy of Th II to Abd V.

(A) Orchesella mesovoides sp. nov. (B) Orchesella colluvialis sp. nov. (bar 0.2 mm; S-chaetae and S-microchaetae drawing in the lateral x10).

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Table 2.

Comparison of characters among the different species of Orchesella cited in this paper.

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Table 3.

Comparison of characters among the different species of Orchesella cited in this paper.

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Fig 9.

Dorsal macrochaetotaxy of Th II to Abd V.

(A) Orchesella villosa. (B) Orchesella cincta (bar 0.2 mm; S-chaetae and S-microchaetae drawing in the lateral x10).

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Fig 10.

Dorsal macrochaetotaxy of Th II to Abd V of Orchesella eolia (bar 0.2 mm).

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Fig 11.

MSS temperature.

Temperature records of two of the SSDs, one (SSD-14) was the highest in Peñalara (Majada Hambrienta) (1994 m a. s. l.), and the other (SSD-7) was the lowest in El Purgatorio (1406 m a. s. l.). x-axis: number of measures from June to September; y-axis: temperature in Celsius degrees.

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Fig 12.

Number of specimens of the two Orchesella species described in this paper ordered by altitude.

(A) Geographical orientation of the vectors of abundance of the two species. (B) Abundance of the species in relation to altitude (orange: O. colluvialis sp. nov.; blue: O. mesovoides sp. nov.).

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Table 4.

Captures for the species O. mesovoides sp. nov. and O. colluvialis sp. nov. in all the traps.

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