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Fig 1.

Workflow of the study showing timeline for sample collection and processing in this study.

*Only M. tuberculosis culture positive samples were retrospectively selected for the current study.

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Fig 2.

Direct, USP and bio-safe USP smear.

USP and bio-safe USP smears show a clear background as compared to direct smear.

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Table 1.

Smear status of culture positive sputum samples.

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Fig 3.

Direct detection of rpoB mutations in bio-safe sputum samples (n = 87) by DNA sequencing.

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Fig 3 Expand