Table 1.
Sequence of Oligionucleotide primers.
Fig 1.
Age associated variation in the proportion of c-kit+ CSCs in ventricular digests from SHR and Wistar rats.
Graphical representation of data expressed as percentage of total cells. (n = 3). Data presented as mean ± SD. Variation was analysed by two way ANOVA followed by Student t-test. (** p<0.01 and * p<0.05 SHR Vs Age matched WST; †† p<0.01 and † p<0.05 WST & SHR of different ages compared to respective 1 week old pups. Two way ANOVA p<0.001).
Fig 2.
Age associated variation in growth characteristics of CSCs cultured from SHR and Wistar rats.
(a) Colony Forming Units representing number of colonies formed (n = 3) (b) Growth kinetics of 18-month old rats represented as cell number*104 (n = 3) (c) Growth rate was calculated as LogN of the ratio of cell number at two fixed time points (n = 3) (d) Population doubling time (PDT) represented as number of days (n = 3) Data presented as mean ± SD. Variation was analysed by two way ANOVA followed by Student t-test. (** p<0.01 SHR Vs Age matched WST; †† p<0.01 and † p<0.05 WST & SHR of different ages compared to respective 1 week old pups. Two way ANOVA p<0.001).
Fig 3.
Age associated variation in migration efficiency of CSCs from WST and SHR.
(a) Representative photograph of migrated CSCs as observed by trans-well assay. (b) Graphical representation of migration ability represented as mean number of cells/field (n = 3) Data presented as mean ± SD. Variation was analysed by two way ANOVA followed by Student t-test. (** p<0.01 SHR Vs Age matched WST; †† p<0.01 and † p<0.05 WST & SHR of different ages compared to respective 1 week old pups. Two way ANOVA p<0.001).
Fig 4.
Age associated variation in ROS levels of CSCs of WST and SHR based on H2DCFDA fluorescence.
Data presented as mean ± SD. (n = 3) Variation was analysed by two-way ANOVA followed by Student t-test. (**p<0.01 and * p<0.05 SHR Vs age matched WST; and †† p<0.01 WST & SHR of different ages compared to respective 1 week old pups. Two way ANOVA p<0.001).
Fig 5.
Age associated variation in cardiovascular differentiation of CSCs from WST and SHR determined by Western blot analysis Representative blots and graphical representation of the expression of (a) Cardiac troponin I and (b) smooth muscle actin (n = 3) Data presented as mean ± SD. Variation was analysed by two way ANOVA followed by Student t-test. (†† p<0.01 and † p<0.05 WST & SHR of different ages compared to respective 1 week old pups. Two way ANOVA p<0.001).
Fig 6.
Age associated variation in the proportion of senescent CSCs in WST and SHR.
(a) Graphical representation of percentage of senescent cells (n = 3) (b) Representative blots and graphical representation of p16ink4a protein expression (n = 3) (c) Representative blots and graphical representation of p21 protein expression (n = 3) Data presented as mean ± SD. Variation was analysed by two way ANOVA followed by Student t-test. **p<0.01 SHR Vs Age matched WST; †† p<0.01 and WST & SHR of different ages compared to respective 1 week old pups. Two way ANOVA p<0.001).
Fig 7.
Age associated variation in expression of TERT mRNA, telomerase activity and level of DNA damage in SHR and Wistar rat.
(a) Graphical representation of TERT mRNA levels expressed as fold change (n = 3) (b) Representative photograph of telomerase activity (c) Graphical representation of telomerase activity (n = 3) (d) Representative photograph of DNA damage as evaluated by Comet assay (e) Graphical representation of DNA damage represented as comet tail moment (n = 3) Data presented as mean ± SD. Variation was analysed by two way ANOVA followed by Student t-test. **p<0.01 SHR Vs age matched WST; †† p<0.01 and † p<0.05 WST & SHR of different ages compared to respective 1 week old pups. Two way ANOVA p<0.001).