Table 1.
Numbers of OTUs of intestinal microbe from hindgut contents of three individuals of L. japonica under different taxonomic level.
Table 2.
The microbial diversity index including Chao1, ACE, Simpson, and Shannon of 16S rRNA sequence library from three individuals of L. japonica at a given rarefied depth.
Fig 1.
The rarefaction and the rank abundance analysis of the microbe species from hindgut contents of L. japonica.
(A) the rarefaction curve; (B) the rank abundance curve.
Fig 2.
Microflora distributions of the microbe species from hindgut contents of L. japonica in five classification levels.
(A) In the phylum level; (B) In the class level; (C) In the order level; (D) In the family level; (E) In the genera level.
Fig 3.
Top 20 dominant microbes in different classification level.
The colored circles imply the top 20 dominant microbes in relative abundance in different classification level. The larger the radius of the circles is, the higher the species in abundance. The non-colored part implies microbes that are not in the top 20 dominant. P: Phylum; C: Class; O: Order; F: Family; G: Genera.
Fig 4.
Hierarchically clustered heatmap analysis of the top 50 genera in intestine of different individuals of lamprey.
The relative values for bacterial genera are indicated by color intensity with the legend indicated at the top right corner of the figure.
Fig 5.
The pairwise comparison of bacterial taxa abundance in intestine between three individuals of lamprey.
(A) top 20 member of bacteria in genera level; (B) top 7 member of bacteria in the phylum level.
Fig 6.
UPGMA analysis clustering analysis based on UniFrac distance matrix.
(A) UPGMA tree based on unweighted unifrac distance matrix; (B) UPGMA tree based on weighted unifrac distance matrix. The UPGMA analysis revealed that gut microbiota derived from L. japonica display little similarity with other aquatic organism of Vertebrata. Lamprey: gut microbiota deep sequence data derived from L. japonica in this study; farmland frog, American alligator, grass carp, and zebrafish: gut microbiota deep sequence data derived from public database.
Fig 7.
The relative abundance of intestinal microbe involving in the subfunction of metabolic pathways predicted through KEGG pathway database.
Fig 8.
Venn diagram showing shared OTUs and shared functional clusters of microbes in different individuals of lamprey.
(A) Shared OTUs; (B) Shared functional clusters of microbes.