Table 1.
Conditions for AAS.
Fig 1.
Viability of E. coli (A) or S. warneri (B) after 3h culture in the presence of textile samples was analyzed by the MTT assay. A viability of 100% corresponds to the signal obtained with bacteria in the absence of any textile samples. Data are shown as means +S.E.M. of n = 6 independent experiments. *Mean significantly different from untreated textile; One-way ANOVA with Tukey´s post-hoc test (p < 0.05). n.d.: not detected.
Fig 2.
To monitor metal release during incubation of the bacteria, textile samples were kept for 3h at 37°C in LB or TYSE media on a rotating incubator. Subsequently, the total amounts of Ag released from polyacrylate coating, thickness 200 μm (A) or Cu released from polyaniline coating containing 5% Cu (B) in the media were quantified by AAS. Data are shown as means +S.E.M. of n = 3 independent experiments.
Fig 3.
Viability of E. coli (A) or S. warneri (B) after 3h culture in the presence of textile samples was analyzed by measuring the optical density at 600 nm. A viability of 100% corresponds to the signal obtained with bacteria in the absence of any textile samples. Data are shown as means +S.E.M. of n = 3 independent experiments. *Mean significantly different from untreated textile; One-way ANOVA with Tukey´s post-hoc test (p < 0.05).
Fig 4.
OD600 assay with background correction.
In a complementary experiment to Fig 2, the effect of leaching of coating from the textile samples after incubation in bacteria-free media for E. coli (LB-medium, A) or S. warneri (TYSE-medium, B) was measured at 600 nm. Original data (monochromatic bars) and data after correction by subtracting the absorption at 600 nm caused by leaching (checkered bars) are shown as means +S.E.M. of n = 3 independent experiments.
Fig 5.
OD600 assay at different time points.
Viability of E. coli cultured in the presence of the indicated concentrations of AgNO3. Data are shown as means +S.E.M. of n = 3 independent experiments.
Fig 6.
The area of the bacteria-free zone around textile disks placed on agar plates coated with E. coli (A) or S. warneri (B) was measured. Data are shown as means +S.E.M. of n = 4 independent experiments. *Mean significantly different from untreated textile; One-way ANOVA with Tukey´s post-hoc test (p < 0.05).
Fig 7.
Viability of E. coli (A) or S. warneri (B) after 3h culture in the presence of textile samples was analyzed by colony formation. A viability of 100% corresponds to the signal obtained with bacteria in the absence of any textile samples. Data are shown as means +S.E.M. of n = 3 independent experiments. *Mean significantly different from untreated textile; One-way ANOVA with Tukey´s post-hoc test (p < 0.05).
Table 2.
Correlation analysis.
Table 3.
Time requirement.
Fig 8.
Viability of E. coli (A) or S. warneri (B) after 3h culture on textile disks was analyzed by colony formation. To illustrate the resolution in the low viability range, values are depicted on a logarithmic scale. Data are shown as means +S.E.M. of n = 3 independent experiments. *Mean significantly different from untreated textile; One-way ANOVA with Tukey´s post-hoc test (p < 0.05). n.d.: not detected.