Fig 1.
Growth of Metridium farcimen injected with 10% neutral red, 10% methylene blue, or seawater (control) in (A, B) laboratory and (C, D) field experiments. There was no significant difference between the marked anemone and control growth rates in either both laboratory or field experiments (p>0.05, n = 21 and n = 10 respectively). Values are means ± standard error. The dashed line in panel C indicates the time at which methylene blue marked individuals lost sufficient color to be identified as marked. Neutral red individuals were still clearly visible at 156 days. Growth rates were compared at 40 and 156 days between all treatments that had visibly marked individuals.
Fig 2.
Metridium farcimen (A) before injection and injected with 1.0 mL of (B) 10% methylene blue, (C) 10% neutral red, and (D) 0.25% fluorescein. Endodermal tissue in the column and tentacles as well as cinclides are clearly marked in stained anemones.
Fig 3.
One polyp of Metridium farcimen one year after injection with neutral red amongst non-injected individuals.