Fig 1.
Frequency of IFNγ secreting PBMC measured in ELISpot assays after stimulation with inactivated pdm09 virus.
(A, D), uCD4i, (B, E), and uCD8i (C, F) epitope library. Top row represents differences among cases and controls (A, B, C), bottom row represents differences between asymptomatic and symptomatic cases (D, E, F). Inlet in figure A zooms on the range of cell frequencies presented on a linear scale (p values, Wilcoxon-Mann-Whitney test).
Fig 2.
CD95+ CD8+ Temra cells as cellular immune correlates of protection against symptomatic ILI after exposure to pdm09 virus in pregnancy.
PBMC samples were stimulated ex vivo, y-axis in all panels shows cell frequency fold change over unstimulated control: (A) CD8+ Temra, (B) CD95+ CD8+ Temra, (C) CD107+ CD8+ Temra (p vlaues, Dunn’s Kruskal-Wallis post hoc test).
Fig 3.
Effector functions of CD4+ and CD8+ T cells.
Median cell frequency fold changes of (A) CD4+, (B) CD8+ T cells after stimulation with the pdm09 virus, and median CD8+ T cell frequency fold change after stimulation with (C) uCD8i, (D) CMV epitopes. Dotted lines in graphs represent the fold change level (p values, Dunn’s Kruskal-Wallis post hoc test).
Fig 4.
Cell frequency fold change among controls, asymptomatic and symptomatic cases after the pdm09 virus stimulation.
(A) total NK cells, (B) CD107a+ NK cells, (C) CCR7+ NK cells, (D) IFNγ+ NK cells, (E) radar graph of specific NK cell populations characterized by the surface markers CD16 and CD56, and positive for CD107a, CCR7 or IFNγ expression. Dotted lines in graph E represent the fold change level (p values, Dunn’s Kruskal-Wallis post hoc test in D, factorial ANOVA in E).