Table 1.
Patient demographics and wound characteristics at baseline (T = 0) and the end of study (T = 4).
Fig 1.
Consort flow-diagram for patient enrollment.
Fig 2.
Bates-Jensen Wound Assessment (BWAT) scores of each wound.
Patient diabetes status is separated by different colors (black = diabetic, light grey = non-diabetic) and the wound type is separated by shape of data point (square = non-leg, circle = lower leg). Open circle or square indicates Baseline (Visit 1) and crosses indicate the last time point measured (Visit 6). Treatment occurs from visit 2-visit 6 (V2-V6). Solid lines indicate the mean and standard deviation of the BWAT score for each subject timeline.
Table 2.
Comparison of median wound score (median BWAT ± IQR) between diabetes status and among wound types before and after treatment.
Median wound score ratio = visit 6/visit 1 ± IQR, Median wound score change = visit 6—visit 1 ± IQR.
Fig 3.
Bacterial communities in swab and debridement samples.
The 249 species level OTUs identified from the specimens are classified according to their corresponding phylum. The venn diagram shows 120 OTUs that are found in both debridement and swab specimens, while 59 OTUs are debridement-specific and 70 OTUs are swab-specific (as indicated by the square). OTUs with at least 2-fold numerical differences in relative abundance (>1% for at least one sample type) between the swab and debridement samples. Allobaculum sp. is more abundant in swab samples, while an undefined Burkholderiales sp. and Roseateles depolymerans are more abundant in debridement samples.
Fig 4.
Overview of the bacterial communities between diabetes status and among wound types in pre-treated samples (V1 samples).
(A) Plots for swab samples. The Y axis represents the percentage of the OTUs being observed among the total reads. (B) Plots for debridement samples. Y axis represents the percentage of the OTUs being observed among the total reads.
Table 3.
Comparison of alpha diversity in pre-treatment (V1) samples.
Fig 5.
PCoA clustering, common/unique bacterial OTUs at species level, and differentially abundant bacterial OTUs between diabetes status and among wound types in post-treatment samples (V2-V6 samples).
(A) Plots for swab samples. Venn diagrams suggest that diabetic status-associated and wound type-associated OTUs exist in swab samples. Differentially abundant OTUs are listed in the bar chart. (B) Plots for debridement samples. Venn diagrams suggest that diabetic status-associated and wound type-associated OTUs exist in debridement samples. Differentially abundant OTUs are listed in the bar chart.
Table 4.
Comparison of alpha diversity in treated (V2-V6) samples.
Fig 6.
Bacterial relative abundance (>1% abundance in all samples) plots for three patient timelines.
Plots are split by debridement samples (top panel) and swab samples (bottom panel). Wound locations are as follows: P10 (post-surgical abdomen, non-F/L wound), P8 (venous leg ulcer), P9 (diabetic foot ulcer). Debridement samples were not taken on visit 2.