Table 1.
Mitochondrial function traits analyses of variance results.
Table 2.
Physiological trait analyses of variance results.
Fig 1.
Complex I structure prediction of Japan mtDNA.
(A) The ND2 region corresponds to the bacterial subunit NuoN. (B) The structure of the proton pump. The dark blue residues that are transparently shaded make up the proton channel. (C) The location of the variant and their isoelectric point. The yellow is the amino acid that is changed in the Japan line. The arrow represents the movement of the TM7 domain.
Fig 2.
The mitochondrial CI-OXPHOS of Drosophila melanogaster harboring Alstonville (Alst) and Japan (Jap) mtDNA in the w1118 genetic background and the Oregon R (OreR) background.
Flies were aged 11 (upper chart) and 25 d (lower chart). The Protein: Carbohydrate (P:C) diets were 1:2, 1:4, 1:8 and 1:16. (A) Males with the w1118 genetic background. (B) Females with the w1118 genetic background. (C) Males with the OreR genetic background. (D) Females with the OreR genetic background. Bar represents oxygen flux per mass, and error bars show the standard error of the mean.
Fig 3.
The mtDNA copy number of Drosophila melanogaster harboring Alstonville (Alst) and Japan (Jap) mtDNA in the w1118 genetic background.
Flies were aged 11 (upper chart) and 25 d (lower chart). The protein: carbohydrate (P:C) diets were 1:2, 1:4, 1:8 and 1:16. (A) Males. (B) Females. MtDNA copy number of females harboring the two mtDNA types did not differ, so they were pooled. Data for each mitotype is presented in S1 Fig. Bar represents mtDNA copy number, and error bars show the standard error of the mean.
Fig 4.
The maximum ROS production of Drosophila melanogaster harboring Alstonville (Alst) and Japan (Jap) mtDNA in the w1118 genetic background.
Flies were aged 11 (upper chart) and 25 d (lower chart). The protein: carbohydrate (P:C) diets were 1:2, 1:4, 1:8 and 1:16. (A) Males. (B) Females. Maximum ROS production of female flies harboring the two mtDNA types did not differ, so they were pooled. Data for each mitotype is presented in S2 Fig. Bar represents basal ROS production, and error bars show the standard error of the mean.
Fig 5.
The SOD activity of Drosophila melanogaster harboring Alstonville (Alst) and Japan (Jap) mtDNA in w1118 genetic background.
Flies were aged 11 (upper chart) and 25 d (lower chart). The protein: carbohydrate (P:C) diets were 1:2, 1:4, 1:8 and 1:16. (A) Males. (B) Females. The SOD activity of females harboring the two mtDNA types did not differ, so they were pooled. Data for each mitotype is presented in S3 Fig. Bar represents SOD activity, and error bars the standard error of the mean.
Fig 6.
The fecundity of Drosophila melanogaster harboring Alstonville (Alst) and Japan (Jap) mtDNA in w1118 genetic background.
The flies were aged from 1–11 d (upper chart) and 12–25 d (lower chart). The protein: carbohydrate (P:C) diets were 1:2, 1:4, 1:8 and 1:16. (A) Males. (B) Females. (C) The fecundity of males (11 d or 25 d) mated with 5 d old virgin females fed on the intermediate 1:6 P:C diet. Data for each mitotype is presented in S4 Fig. Bar represents total egg count, and error bars show standard error of the mean.
Fig 7.
The survival of Drosophila melanogaster harboring Alstonville (Alst) and Japan (Jap) mtDNA in w1118 genetic background.
The protein: carbohydrate (P:C) diets were 1:2, 1:4, 1:8 and 1:16. (A) Males. (B) Females. Survival of females harboring the two mtDNA types did not differ, so they were pooled for graphical presentation. Data for each mitotype is presented in S5 Fig. Bar represents 50% survival, and error bars show standard error of the mean.
Fig 8.
The lipid content of Drosophila melanogaster harboring Alstonville (Alst) and Japan (Jap) mtDNA in w1118 genetic background.
Flies were aged 11 d (upper chart) and 25 d (lower chart). The protein: carbohydrate (P:C) diets were 1:2, 1:4, 1:8 and 1:16. (A) Male. (B) Female. Lipid content of females harboring the two mtDNA types did not differ, so they were pooled. Data for each mitotype is presented in S6 Fig. Bar represents lipid content, and error bars show standard error of the mean.
Fig 9.
The starvation resistance of Drosophila melanogaster harboring Alstonville (Alst) and Japan (Jap) mtDNA in w1118 genetic background.
Flies were aged 11 (upper chart) and 25 d (lower chart). The protein: carbohydrate (P:C) diets were 1:2, 1:4, 1:8 and 1:16. (A) Males. (B) Females. Bar represents 50% survival, and error bars show standard error of the mean.