Fig 1.
Map of the investigated area with marked sampling localities.
1- Lergue, 2-Hérault, 3-Argens, 4-Var, 5-Soča, 6-Gradaščica, 7-Jevnica, 8-Bregana, 9-Roya, 10-Bevera, 11-Staffora, 12-Po, 13-Arno, 14-Tiber, 15-Volturno, 16-Jasenak field, 17-Sušik, 18-Studenac, 19-Šmit lake, 20-Jadova, 21-Ričica, 22-Suvaja, 23-Krbava, 24-Čikola, 25-Vinelić, 26-Cetina, 27-Gatačko field, 28-Dabarsko field, 29-Konavle, 30-Zeta, 31-Morača, 32-Cijevna, 33-Kalamas, 34-Pinios, 35-Pavllo, 36-Louros, 37-Acheloos, 38-Evinos, 39-Alfios, 40-Kifissos. Different colors represent ranges as used for S-DIVA analysis.
Table 1.
Sampling localities and number of specimens belonging to certain species collected at each locality.
Fig 2.
Phylogenetic tree of cyt b sequences based on Bayesian (BAY) and Maximum parsimony (MP) inferences.
Numbers at nodes represent Bayesian posterior probabilities and MP branch support.
Fig 3.
Phylogenetic tree of RAG1 sequences based on Bayesian (BAY) and Maximum parsimony (MP) inferences.
Numbers at nodes represent Bayesian posterior probabilities and MP branch support. Asterisk denotes one allele from the TEEV7 sample, which belongs to Squalius sp.
Fig 4.
Median-joining network of nuclear haplotypes.
Black circles represent median vectors. The number of mutational steps is displayed by the branches if higher than 2. Haplotype clusters are marked with roman numbers and haplotypes belonging to different species are presented with different colors. The arrow marks one allele from the TEEV7 sample, which belongs to Squalius sp.
Fig 5.
Divergence time estimations inside the genus Telestes based on cyt b sequences.
Timing of the splitting events is presented by mean values and the 95% credibility range (in brackets), in million years ago. Numbers in square brackets are posterior probabilities (*-posterior probability = 1).
Fig 6.
Divergence time and ancestral geographic ranges estimations inside the genus Telestes based on the concatenated data set (cyt b and RAG1).
Timing of the splitting events is presented by mean values and the 95% credibility range (in brackets), in million years ago. Numbers in square brackets are posterior probabilities (*-posterior probability = 1). Ranges—A: rivers in the NW part of the Mediterranean area (Lergue, Hérault, Argens, Var, Roya, Bevera, Stafora, Po), B: Apennine Peninsula (Arno, Tiber, Volturno), C: rivers in northern Dinarids belonging to the Black Sea drainage (Soča, Gradašćica, Jevnica, Bregana, Jasenak field, Sušik, Studenac, Šmit lake), D: karstic rivers in middle Dinarids (Jadova, Ričica, Suvaja, Krbava, Čikola, Vinelić, Cetina, Gatačko field, Dabarsko filed, Konavle), E: Adriatic rivers in Monte Negro (Zeta, Morača, Cijevna), F: rivers of southern Albania and Greece (Pavllo, Kalamas, Louros, Acheloos, Evinos, Alfios, Kifissos).
Table 2.
Ranges and mean values (in brackets) of the p-distances among species of the genus Telestes, based on cyt b (regular letters) and RAG1 (bold letters) genes.
Table 3.
Ranges and mean values (in brackets) of the intraspecific p-distances of Telestes species.
Table 4.
Genetic polymorphism measures of Telestes species in cyt b and RAG1 genes.
Fig 7.
Colonization routes of the Telestes lineages, based on the proposed evolutionary scenario.
*—Telestes ancestor in 7a and 7b, ancestor of T.croaticus/T. fontinalis in 7c and 7d; #—ancestor of T. souffia; x–ancestor of the species cluster (T. metohiensis/T. dabar/T. miloradi/T. muticellus/T. montenigrinus/T. ukliva/T. turskyi/T. karsticus/T. polylepis); o–ancestor of T. pleurobipunctatus/T. beoticus.