Fig 1.
The disease cycle of pine wilt associated with pine trees, nematodes, and beetles.
The center circle indicates seasons, although the length of the arc does not correspond to the actual span of the seasons in Japan.
Fig 2.
The life cycles of the pine wood nematode and Caenorhabditis elegans.
In addition to regular ‘propagative’ (microbe-feeding) stages, each has ‘dispersal (JIII and JIV)/dormant (J2d and Dauer)’ stages.
Fig 3.
Propagation and JIII production of four field isolates of Bursaphelenchus xylophilus after 10, 20, and 30 days of incubation.
A: total number of nematodes; B: number of JIII; C: JIII proportion. The same letters indicate non-significant differences between the samples at the same time point. Bars and error bars represent averages and standard errors for ten replicates, respectively.
Fig 4.
Propagation and JIII production of eight inbred lines of Bursaphelenchus xylophilus derived from the T4 isolate after 10, 20, and 30 days of incubation.
A: total number of nematodes; B: number of JIII; C: JIII proportion; D: correlation between number of total nematodes and JIII proportion. The same letters indicate non-significant differences between the samples at the same time point (A–C). Bars and error bars represent averages and standard errors for ten replicates, respectively (A–C).
Fig 5.
Correlation between number of total nematodes and JIII proportion.
Each plot represents a culture plate.
Fig 6.
Propagation and JIII production of the ST2 line of Bursaphelenchus xylophilus by adding CDBX and various concentrations of yeast (Saccharomyces cerevisiae) after five days of incubation.
A: total number of nematodes; B: number of JIII; C: JIII proportion. The same letters indicate non-significant differences between the samples. Bars and error bars represent averages and standard errors for three replicates, respectively.