Table 1.
Primers for semi qRT-PCR.
Fig 1.
Cordycepin production in the LSC and the SMC of C. militaris NBRC103752.
C. militaris cells were cultivated in the LSC and the SMC, and the concentration of cordycepin was measured every two days from the 3rd d to the 19th d of culture. Circles: LSC, triangles: SMC.
Table 2.
GO term enrichment analysis between LSC and SMC of C. militaris.
Fig 2.
Differentially expressed enzymes of purine nucleotide metabolism in the LSC and the SMC of C. militaris NBRC103752.
Numbers in parentheses indicate the upregulated or downregulated LogFC value. Round dotted, dashed, and long dashed lines denote precursors, different pathways, and proposed cordycepin biosynthesis, respectively. PRPP: phosphoribosyl pyrophosphate, GAR: glycinamide ribonucleotide, FGAR: phosphoribosyl-N-formyl glycine amide, FGAM: 5’-phosphoribosylformylglycinamidine, AIR: aminoimidazole ribonucleotide, GABA: γ-aminobutyric acid, AICAR: 5-Aminoimidazole-4-carboxamide ribonucleotide, AMP: adenosine monophosphate, Asp: aspartate, CAIR: 1-(5-phospho-D-ribosyl)-5-amino-4-imidazolecarboxlate, IMP: inosine monophosphate, SAICAR: phosphoribosyl aminoimidazole-succinocarboxamide.
Table 3.
Differentially expressed genes in purine nucleotide biosynthesis and related pathways of LSC and SMC of C. militaris.
Table 4.
Differentially expressed genes related to the γ-aminobutyric acid (GABA) shunt, pentose phosphate pathway (PPP), glycolysis, and tricarboxylic acid cycle (TCA).
Fig 3.
Semi qRT-PCR of each unigene in the LSC and SMC of C. militaris.
RNA was extracted from mycelia cultivated for 5 d and 13 d in the LSC and the SMC, respectively. The ubiquitin carrier protein gene was used for the normalization of each gene expression. 1: Adenylosuccinate synthase; 2: Adenylosuccinate lyase; 3: Adenosine deaminase; 4: SAICAR synthase; 5: 5’-Nucleotidase; 6: IMP cyclohydrolase. Bars represent means ± 95% confidence intervals.
Fig 4.
Effect of aspartate on cordycepin production in the LSC of C. militaris NBRC103752.
Aspartate at each concentration was added to the culture medium, and the cultures were carried out for 19 d. The cordycepin concentration was measured every two days from 3 d to 19 d. The added aspartate concentrations were 0 g/L (closed circles), 0.5 g/L (open circles), 1.0 g/L (closed squares), and 1.5 g/L (open squares). The values are the results of the three technical repetitions (mean ± SD).