Fig 1.
TEER (A) and permeability to Lucifer yellow (B) in ECV304 and bEnd3 monoculture and co-culture models with C6 cells. ECV304 demonstrated higher TEER and lower permeability to Lucifer yellow than bEnd3. However, a co-culture of ECV304 or bEnd3 with C6 cells resulted in the decrease of TEER and increase of permeability to Lucifer yellow. Data represent means ± SD (n = 3). * p < 0.05.
Fig 2.
Immunofluorescent staining of tight junction proteins occludin, claudin-5 and ZO-1 in ECV304 and bEnd3 cells.
The immunofluorescence of ZO-1 gave distinct strands on cell membrane while the staining of occludin and claudin-5 were diffused and weak in both cell lines. The confocal images were acquired at 20 × magnification.
Fig 3.
R123 uptake in ECV304 and bEnd3 cells in the absence or presence of P-gp inhibitor verapamil.
The P-gp inhibitor verapamil delivered no significant effects on R123 uptake in ECV304 cells but significantly increased R123 uptake in bEnd3 cells in comparison with that in the absence of the inhibitor. Data represent means ± SD (n = 3). ** p < 0.01.
Table 1.
Permeability coefficient values of reference compounds measured in ECV304 and bEnd3 monoculture models.