Table 1.
Histopathological evaluation of liver tissue and results of IHC, serology and real-time PCR in HEV-positive wild boar.
Fig 1.
Histopathologic lesions in wild boar naturally infected with hepatitis E virus.
Fig 1A: liver with presence of mild intralobular lymphoplasmacytic aggregates in the hepatic parenchyma. Inset showing a lymphoplasmacytic aggregate at higher magnification (hematoxylin and eosin, HE). Fig 1B: liver showing slight perilobular infiltrations of lymphocytes (arrowheads) (HE). Fig 1C: hepatic lobule with moderate hyperemia of liver sinusoids (HE).
Fig 2.
Representative photomicrographs of liver sections from 3 wild boar naturally infected with hepatitis E virus.
Fig 2A: liver with absence of association of the HEV antigen inside the lymphoplasmacytic aggregates (Immunohistochemistry (IHC) with the avidin-biotin-peroxidase complex (ABC) method counterstained with hematoxylin). Fig 2B: hepatitis E virus (HEV) immunolabeling in the cytoplasm of the Kupffer cells (arrowhead) and the sinusoidal endothelial cells (arrow) (IHC with ABC method). Fig 2C: HEV immunostaining of a light lymphoplasmacytic infiltrate (arrowhead) (IHC with ABC method). Fig 2D: liver showing slight labeling for HEV antigen in the hepatic parenchyma, which is appreciable in the Kupffer cells (inset) (IHC with ABC method).