Fig 1.
Identification of direct targets of Olig2 in the adult spinal cord in vivo.
(A) Heatmap and line plot representation of ChIP-Seq signal density for Olig2 ChIP and Input Control centered on predicted TSS. (B) Pie chart showing proportions of genomic landmarks corresponding to Olig2-bound targets. (C) Integrative Genomics Viewer (IGV) visualization of Olig2 occupancy at selected target sites. (D) Pie chart showing proportions of targets with protein-coding or non-protein-coding designation. (E) De novo motif analysis of Olig2-binding regions identified a putative E-box motif (CANNTG) among ~60% of all Olig2-bound target regions. This motif configuration is consistent with previously published motif analyses for Olig2-occupancy via ChIP-Seq.
Fig 2.
Functional GO terms overrepresented in the Olig2 ChIP-Seq dataset.
Shown are the highest ranking terms for (A) Biological Processes, (B) Protein Class, and (C) Pathways according to PANTHER (geneontology.org) and ranked according enrichment score (FE; red bars) and statistical significance (-log2p-val; black line) as indicated.
Fig 3.
Validation of ChIP-Seq targets by ChIP-qPCR.
(A) Box plot depicting enrichment over total genomic input (%Input) for both Olig2 ChIP (Olig2) and mock IgG (IgG) control conditions for 13 gene targets identified by ChIP-Seq. (B) IGV genome browser tracks showing the location of primers targeting either a specific ON-target peak summit (green bar; ON) or OFF-target region located 10–30 kb from the nearest peak (red bar; OFF) for Nkx2.2, Opalin, and Zcchc24. Results for all ChIP-qPCR data were generated from three independent experiments.