Table 1.
Initial feed used in the production of chicks up to 30 days of age (ROSTAGNO, GOMES, 2011).
Table 2.
Description of G. gallus genes related to heat stress, reference genes for chickens and their specific primers used in RT-qPCR analyzes.
The primers of HSF3, HSP70 and HSP90 genes were designed by ALMEIDA, (2007).
Table 3.
Parameters of the specific primers of genes related to thermal stress and reference genes for broilers obtained from the analysis of efficiency curve in RT-qPCR.
Fig 1.
Regression curves, amplification and dissociation of the efficiency test for the 4 target genes (HSF1, HSF3, HSP70 and HSP90) from broilers derived from RT-qPCR reactions.
All dissociation curves show only one peak.
Table 4.
Descriptive statistics and expression levels of target genes related to heat stress in broilers (n = 36).
Table 5.
Relative expression analysis of HSF1, HSF3, HSP70 and HSP90 genes in the different genetic groups of chicken comparing the comfort and thermal stress environments.
Fig 2.
Relative expression analysis of HSF1, HSF3, HSP70 and HSP90 genes in different chicken genetic groups comparing comfort and thermal stress environments.
*p-Value <0.05.
Table 6.
Relative expression analysis of HSF1, HSF3, HSP70 and HSP90 genes comparing the different chicken genetic groups within the thermal stress environment.
Fig 3.
Relative expression analysis of HSF1, HSF3, HSP70 and HSP90 genes comparing the different chicken genetic groups within the thermal stress environment.
*p-Value <0.05.
Table 7.
Relative expression analysis of HSF1, HSF3, HSP70 and HSP90 genes comparing the different chicken genetic groups within the thermal comfort environment.
Fig 4.
Relative expression analysis of HSF1, HSF3, HSP70 and HSP90 genes comparing the different genetic groups of chicken within the thermal comfort environment.
*p-Value <0.05.
Table 8.
Relative expression analysis of HSF1, HSF3, HSP70 and HSP90 genes comparing the different genetic groups of chicken without considering the environments (comfort and thermal stress).
Fig 5.
Relative expression analysis of HSF1, HSF3, HSP70 and HSP90 genes comparing the different genetic groups of chicken without considering the environments (comfort and thermal stress).
*p-Value <0.05.