Fig 1.
Ionic cross-linking of sodium alginate sol in contact with calcium ions.
Fig 2.
Schematic workflow of alginate beads production by internal gelation. Using emulsification and an internal initiated gelation to produce beads in a mean size of 200–500 μm.
Fig 3.
200–400 μm sized alginate beads.
Light optical microscope picture of 200–400 μm sized alginate beads produced by internal gelation.
Fig 4.
Schematic workflow of alginate beads production by ultrasonic activation.
Fig 5.
Light optical microscope picture of 50 μm sized alginate particles produced by ultrasonic activation.
Table 1.
Content of artificial saliva.
Table 2.
Calibration standards of HPLC.
Table 3.
Samples for initial screening.
Table 4.
Sample definition of alginate and PLGA beads.
Table 5.
Influence of different CHX-concentrations.
Table 6.
Influence of different loading times.
Table 7.
Combinations of different particle sizes.
Fig 6.
CHX release profiles of different sized alginate beads.
Cumulative CHX release of initial screening measurements (HPLC) over 120 h (n = 1 samples each). CHX release was determined from different sized alginate based particles.
Fig 7.
CHX release profiles of different biodegradable materials.
Cumulative CHX release of 50 μm sized PLGA and 50 μm sized alginate particles over 630 h (mean ± SD, n = 5 samples each).
Fig 8.
CHX release profiles of different CHX concentrations.
Cumulative CHX release of over 630 h (n = 5 samples each). Loading 50 μm sized alginate based particles for 24h in 10% and 20% concentrated CHX. (mean ± SD, n = 5 samples each).
Fig 9.
Initial CHX release profiles of different CHX concentrations.
Cumulative CHX release of initial screening measurements (HPLC) over 20 h. Detailed view on the initial releasing profile of different loaded (10% and 20% CHX) particles. (mean ± SD, n = 5 samples each). (Compare Fig 8).
Fig 10.
Initial CHX release profiles of different loading times.
Cumulative CHX release over 35 h from 200–400 μm sized alginate beads loaded for 1 min, 30 min, 6 h and 24 h and compared with Periochip and Chlosite (mean ± SD, n = 5 samples each).
Fig 11.
CHX release profiles of different loading times.
Cumulative CHX release over 400 h from 200–400 μm sized alginate beads loaded for 1 min, 30 min, 6 h and 24 h and compared with Periochip and Chlosite (mean ± SD, n = 5 samples each).
Fig 12.
Normed CHX release profiles of different loading times.
Normed cumulative CHX release over 400 h and 20 h from 200–400 μm sized alginate beads loaded for 1 min, 30 min, 6 h and 24 h and compared with Periochip and Chlosite (n = 5 samples each).(mean ± SD, n = 5 samples each).
Fig 13.
CHX release profiles of bead combinations.
Cumulative CHX release over 400 h from alginate bead combinations, sized 50 μm and 200–400 μm in ratio 30/70 w/w and 50/50 w/w compared with Periochip (mean ± SD, n = 5 samples each).
Fig 14.
Initial CHX release profiles of bead combinations.
Cumulative CHX release over 20 h from alginate bead combinations, sized 50 μm and 200–400 μm in ratio 30/70 w/w and 50/50 w/w compared with Periochip (mean ± SD, n = 5 samples each).