Table 1.
Validation of microarray by qPCR.
Fig 1.
Representative histopathological photomicrograph of heart lesions in pigs infected with E. rhusiopathiae strain SE38, G4T10 and control.
(A) Heart of a SE38-infected pig with endocarditis and neutrophil infiltration. (B) Thrombogenesis, myocardial necrosis, and inflammatory cell infiltration. (C) Heart of a G4T10-infected pig at 200 and 50μm. (D) PBS control at 200 and 50 μm.
Table 2.
Results of culture and PCR analysis for three pigs challenged with E. rhusiopathiae presented as the number of positive pigs/total pig samples.
Fig 2.
Clustering and characterization of the differential expression of genes.
(A) SE38 group vs. PBS group. S95H_NS, S50H_NS and S96_H_NS belong to SE38 group; S32H_NS, S32H_2_NS and S71H_NS belong to PBS group. DE genes that showed clear functional annotation at 4 dpi between the SE38 and PBS groups were selected for cluster analysis as described in methods. At 4 dpi, a set of 262 genes were upregulated and the remaining 132 genes were downregulated. (B) G4T10 group vs. PBS group. S98H_NS, SR_1_H_NS and SR_2_H_NS belong to G4T10 group; S32H_NS, S32H_2_NS and S71H_NS belong to PBS group. Each row represents a separate gene and each column represents a separate piglet. Red indicates the increased gene expression levels; green denotes the decreased levels compared with normal samples.
Table 3.
DE genes analysis base on KEGG in SE38 and PBS group.
Fig 3.
STRING analysis of the relationship between DE genes.
(A) the DE genes in piglets infected ER were analyzed using STRING database. (B) the network of DE genes related to TLR4.