Fig 1.
Schematic diagram of the LSPR method using aptamer-functionalized for the detection of ABA.
Fig 2.
(A) TEM image of citrate-stabilized AuNPs and (B) the diagram of corresponding particle-size distribution.
Fig 3.
The LSPR absorption spectra of AuNP solutions obtained from the different reagents-added samples: a, aptamer+NaCl; b, aptamer+ABA (5 μM) + NaCl; c, NaCl.
Inset: The corresponding photos of AuNP solutions with different samples.
Fig 4.
The LSPR absorption spectra of detecting target ABA using the whole aptamer (A) and the split aptamer (B) in the absence (a) and presence of ABA (b).
Inset: The absorbance ratio at 620 nm and 520 nm (A620/A520) in the absence and presence of ABA.
Fig 5.
UV-Vis absorption spectra of aptamer-AuNP solution obtained from different concentrations of ABA samples.
Experimental conditions: CNaCl = 100 mM, Captamer = 60 nM.
Fig 6.
Calibration curve established from the absorbance ratio (A620/A520) using different concentrations of ABA samples.
Experimental conditions were the same as in Fig 4.
Fig 7.
Selectivity of this LSPR method for detection of ABA.
Experimental conditions were the same as in Fig 4. ABA: 2 μM; Other compounds: 10 μM.
Table 1.
Analytical results of ABA in fresh leaves of rice.