Fig 1.
Images of immunohistochemical staining for PRCC-TFE3 RCC.
(A) Strong nuclear immunostaining for TFE3 protein (×100); (B) The neoplastic cells showed diffuse cytoplasmic labeling for cathepsin K (×100). RCC, renal cell carcinoma; TFE3, transcription factor E3; H&E, hematoxylin and eosin.
Fig 2.
Images of the PRCC-TFE3 Dual-Fusion FISH assay.
(A) Photomicrograph showed a pair of split red signals and a green signal (negative result, red and green arrows) in each nucleus in male (×1000); (B) Photomicrograph showed a pair of split red signals and two split green signals (negative result, red and green arrows) in each nucleus in female (×1000); (C) A positive result included 2 fused and 1 red signals (yellow and red arrows) in each nucleus in male, indicating that PRCC-TFE3 fusion genes have been formed (×1000); (D) A positive result of female patient showed 2 fused, 1 green and 1red signals (yellow, green and red arrows) in each nucleus (×1000). FISH, fluorescence in situ hybridization.
Fig 3.
Images of microscopic morphology for PRCC-TFE3 RCC.
(A) Photomicrograph showed solid nested architecture composed of compactly arranged, eosinophilic cells (H&E,×100); (B) Tumor cells showed irregular nuclei, inconspicuous nucleoli, eosinophilic cytoplasm, and indistinct cell borders (H&E,×200); (C) Photomicrograph showed histopathology of neoplasm containing clear to eosinophilic cells with voluminous cytoplasm arranged in a papillary pattern(H&E,×100); (D) Photomicrograph showed acinar pattern composed of compactly arranged, clear to slightly eosinophilic cells, and a psammoma body(arrow)(H&E,×100); RCC, renal cell carcinoma; H&E, hematoxylin and eosin.
Table 1.
The data of clinicopathologic features, TFE3 and cathepsin K IHC, PRCC-TFE3 FISH assay of PRCC-TFE3 renal cell carcinomas.