Fig 1.
Tissue-specific expression of trio rescues pupation in the seq-enhanced trio mutant background.
The percent of expected pupae was determined for the following genotypes: trio-mutant, trio-mutant background (trioM89 / trioP1); seq-enhanced, seq-enhanced trio mutants (seq9.17 / +; trioM89 / trioP1); R-pan neural, rescue by pan-neural trio expression in the seq-enhanced trio mutant background (GAL4-elav.L / seq9.17; trioM89 / trioP1, UAS-trio.B); R-class IV da, rescue by class IV da trio expression in the seq-enhanced trio mutant background (GAL4-ppk1.9, UAS-mCD8GFP / seq9.17; trioM89 / trioP1, UAS-trio.B). N = 15 for all genotypes. ANOVA p-value <0.0001; *** indicates pair-wise statistical significance at p<0.001 as compared to *seq*-enhanced. R-pan neural and R-class IV da are also statistically different from each other at p<0.001. Error bars represent the standard error of the means.
Fig 2.
Representative ddaC morphologies in control, mutant and rescued third instar larvae.
(A) ddaC in wildtype larvae (GAL4-ppk1.9, UAS-mCD8GFP / +; trioM89 / +, image shown is 300.8 μm x 650.5μm). (B) ddaC in simple trio mutant larvae (GAL4-ppk1.9, UAS-mCD8GFP / +; trioM89 / trioP1, image shown is 302.8 μm x 749.8μm). (C) ddaC in seq-enhanced trio mutants (GAL4-ppk1.9, UAS-mCD8GFP / seq9.17; trioM89 / trioP1, image shown is 300.4 μm x 601.7μm). (D) ddaC in ppk1.9-trio rescued seq-enhanced trio mutant larvae (GAL4-ppk1.9, UAS-mCD8GFP / seq9.17; trioM89 / trioP1, UAS-trio.B, image shown is 351.1 μm x 751.2μm). The dorsal midline runs horizontally through the approximate center of each image. In (A) representative examples of iso-neuronal avoidance defects are indicated with yellow triangles while representative examples of hetero-neuronal tiling defects are indicated with red triangles. Scale bars show 100 μm.
Fig 3.
Quantification of ddaC dendrite morphologies in control, mutant and rescued third instar larvae.
Genotypes in all panels: W, wildtype larvae, N = 9; B, trio-mutant background, N = 8; E, seq-enhanced trio mutants, N = 9; R, class IV da trio expression in the seq-enhanced trio mutant background, N = 10. Error bars represent the standard error of the means. (A) Dendritic density (dendrite length in μm/μm2 surface). ANOVA p-value = 0.0005; E and R are not statistically different from each other, but both are statistically different from both W and B at p<0.05. (B) Dendritic branch frequency (dendritic branches/dendrite length in μm). ANOVA p-value = 0.0022; E is statistically different than W, B and R at p<0.05. (C) Iso-neuronal avoidance defects (iso-neuronal avoidance defects/dendrite length in μm). ANOVA p-value = 0.086. (D) Hetero-neuronal tiling defects (hetero-neuronal tiling defects/μm of path length of interface between dendritic fields). ANOVA p-value = 0.197.
Table 1.
Mechanical nociception in control, mutant and rescued third instar larvae.