Fig 1.
Electrophoretic analysis of WGA libraries by Agilent 2200 TapeStation Instrument.
The libraries were obtained by DOP-PCR (lane 1), PicoPlex (lane 2), and iDOP-PCR (lane 3) from 15 pg of human gDNA. M–DNA marker “Genomic DNA ScreenTape”.
Table 1.
Multiplex STR genotyping of WGA samples and non-amplified gDNA.
Table 2.
Comparison by NGS the parameters of PicoPlex and iDOP-PCR whole-genome amplification of single-genome-copies.
Fig 2.
Lorenz curves of PicoPlex and iDOP-PCR WGA samples.
A Lorenz curve gives the cumulative fraction of reads as a function of the cumulative fraction of genome. Perfectly uniform coverage would result in a diagonal line (black). PicoPlex (red curve) and iDOP-PCR (blue curve) generate similar deviations from the diagonal as a result of biased coverage. All samples were sequenced at 8x depth.
Fig 3.
CNVs of diploid human genome from single genome copies amplified by PicoPlex and iDOP-PCR WGA methods.
Digitized copy numbers across the genome are plotted for two PicoPlex and two iDOP-PCR WGA samples as well as the non-amplified gDNA sample for control. Raw data at a sequencing depth of 8× with a bin size of 1,000 kb are mapped to the human reference genome. The chromosomes are shown in alternating red and blue colors.