Fig 1.
Flow chart of study conduct.
Fig 2.
Ex-vivo OCT cross-sectional image visualizing the different layers of the human airway wall and corresponding histology image.
(A) Histology cross section, stained with H&E. (B) Higher magnification view of the square of histology image A, visualizing the different layers of the airway wall of the segmental LLL. (C) Histology cross section, stained with desmin. (D) Higher magnification view of the square of histology image C, visualizing the submucosal muscular layer of the airway wall. (E) Corresponding cross section of OCT of ex-vivo airway to histology airway image A and C. (F) Higher magnification view of the square of OCT image E, visualizing the corresponding layers of the airway wall.
Fig 3.
Ex-vivo OCT cross-sectional image and corresponding histology image of human airway.
(A) Clean histology cross section of human airway of the segmental LLL, stained with desmin. (B) cross section images of histology, stained with desmin, with manually traced perimeters; PL: lumen perimeter, Pmuc: mucosal perimeter, Psubmusc: submucosal muscular perimeter. (C) Corresponding cross section of OCT of ex-vivo airway to histology airway image A. (D) cross section images of OCT and with manually traced perimeters; PL: lumen perimeter, Pmuc: mucosal perimeter, Psubmusc: submucosal muscular perimeter and OCT probe in situ.
Table 1.
Patient characteristics undergoing OCT and lobectomy.
Table 2.
Correlation between ex-vivo OCT and histology for airway wall area measurements.
Fig 4.
Linear regression analysis and Bland-Altman plots for histology and OCT ex-vivo airway wall area measurements (n = 51).
(A) AL lumen area in mm2. (B) Amuc mucosal area in mm2. (C) Asubmusc submucosal muscular area in mm2. (D) WAt total airway wall area in mm2. (E) WAmuc mucosal wall area in mm2. (F) WAsubmusc submucosal muscular wall area in mm2.