Fig 1.
Extraction techniques contribute to differences in dynamic range of relative telomere length.
(Top) Dynamic range of RTL (standardized T/S ratio) by extraction technique in matched samples from the same subjects, median marked by black bar and (Bottom) count of samples, median standardized T/S ratio, range of standardized T/S ratio, and p-value for Wilcoxon signed rank test for paired samples by each extraction technique.
Fig 2.
Correlation of relative telomere length (standardized T/S ratio) of matched subjects across extraction techniques and assay techniques.
Inset heat map displays coefficient of determination (R2) for each correlation.
Fig 3.
Correlation of relative telomere length (standardized T/S ratio) of matched samples pre- and post-purification.
(Top) All samples by purification technique. (Bottom) By purification technique and extraction technique, shown by color, for 10 matched subjects extracted using three different techniques.
Fig 4.
Correlation of relative telomere length (standardized T/S ratio) of same samples after 6 months at various concentrations and storage temperature conditions.
(a) 1 ng/uL at 4°C, (b) 1 ng/uL at -30°C, (c) 25 ng/uL at 4°C, and (d) 25 ng/uL at -30°C.