Table 1.
Primers used for PCR in this study.
Fig 1.
Growth of H. pylori strains isolated from IDA or control patients.
Each strain was inoculated into Brucella broth containing 5% horse serum at an initial OD595 0.05. The growth of H. pylori was measured (OD595) by microplate photometer after 24-hour incubation. The Mean±SD 24-hour growth value was measured based on three independent determinations for each strain. Bacterial growth was not significantly different between the IDA and control strains (p = 0.17; Wilcoxon rank sum test).
Fig 2.
Hierarchical cluster analysis of gene expression alteration of control H. pylori strains under iron-replete and limited conditions.
Each strain was cultured with DFM (iron-limited condition, indicated as TH3-F, TH7-F, TH4-F, and TH6-F) or without DFM (iron-replete condition, indicated as TH3, TH7, TH4, and TH6). The genes with significant differences in expression were included in hierarchical clustering analysis using average linkage and Euclidean dissimilarity methods. Genes with a Fur-binding site consensus sequence are indicated in pink.
Table 2.
Expression of H. pylori genes known to be related to iron metabolism.
Fig 3.
Transcription of sabA gene in IDA or control strains by real-time RT-qPCR.
Panel A: overall expression (relative quantitation) of sabA in the four IDA and four control strains, respectively. Panel B: expression (relative quantitation) of sabA in the eight strains studied each. Expression of sabA was normalized in relation to house-keeping gene gyrA. For relative quantitation, H. pylori TH5 (control) strain was used as control, because expression value of the strain was the least (defined as 1.0). Panel A: results are shown as the Mean ± SE for triplicate determinations. ** p = 0.029, using Wilcoxon rank sum test.
Table 3.
H. pylori genes with higher expressiona in IDA strains.
Table 4.
H. pylori genes with lower expression in IDA strains.
Fig 4.
VacA activity in IDA and control strains.
Panel A: H. pylori cytotoxic activity. Filtrate obtained from 72-hour cultures of each H. pylori strain was serially diluted and added to AGS cell cultures. After 24-hour incubation, the final dilution with visible vacuoles was determined microscopically and reciprocal number of the dilution used for calculation. Panel B: Immunoblot analysis of culture filtrate of the eight H. pylori strains.
Table 5.
Expression of H. pylori OMPs and genes associated with host interaction.