Table 1.
Pathological and clinical characteristics of patients, according lamin A expression.
Fig 1.
Expression pattern of lamins in lung adenocarcinoma cells from metastatic pleural effusions.
(A) Representative results of western blot analysis of nuclear extracts from patients (Pt) 5, 9, 10, 11, and 16 and from total proteins extracts of control dermal fibroblasts using a mouse anti-lamin A/C antibody (Jol2). (B) Analyses of lamin A levels quantified after normalization with the total amount of lamins A and C showed two lamin A expression groups (p<0.001; Mann–Whitney test).
Fig 2.
Immunofluorescence staining of lamins A and C in cells from metastatic pleural effusions.
Cells from metastatic pleural effusions of lung adenocarcinoma from high (Patient (Pt) 51) and low (Pt 58) lamin A expression group were stained with lamin A and lamin C antibodies. DAPI: counterstained nuclei. White stars: dyskaryotic nuclei of cancer cells. Orange arrows: cancer cells with a high lamin A expression. White arrows: cancer cells with a low lamin A expression. Asterisk: normal leukocytes.
Fig 3.
Flow cytometry analysis of lamin A and EMA in adenocarcinoma cells.
(A to E) Representative results of lamin A expression in malignant cells contained in 2 metastatic pleural effusions from lung adenocarcinoma (left panel = Patient (Pt) 16 and right panel = Pt 45) using flow cytometry. Positivity thresholds were defined using isotype controls. Cell analysis strategy: (A) Cells were selected using SSC and FSC criteria. (B) Live cells lacking CD45 expression were considered adenocarcinoma cells (CD45-/EMA- and CD45-/EMA+ cells). Leukocytes (CD45+/EMA- cells) were excluded from analysis. (C and D) Lamin A and EMA expression in adenocarcinoma cells. See also Table 2.(E) Correlation of EMA and lamin A expression in adenocarcinoma cells: The percentage of lamin A-expressing adenocarcinoma cells was positively correlated with the percentage of EMA-expressing cells (p = 0.0123; Spearman test).
Table 2.
Lamin A and EMA expression in adenocarcinoma cells.
Fig 4.
Correlation between Lamin A expression in metastatic lung adenocarcinoma cells and number of metastatic sites and WHO Performance status.
(A) The number of metastasis sites was inversely correlated with lamin A expression (p = 0.04; Pearson test). (B) The patients repartition was statistically different according the lamin A expression group (p = 0.037; Chi-square test). PS = Performance status. (C) The WHO Performance status was inversely correlated with lamin A expression (p = 0.01; Spearman test).