Table 1.
Genome comparisons of sequenced Rathayibacter toxicus strains.
Fig 1.
Collinearity of four complete R. toxicus genomes.
A Mauve alignment shows two large locally collinear blocks separated by short transpositions. Green line connects short transposed region. A) R. toxicus FH-79; B) R. toxicus FH-232; C) R. toxicus FH-145 (NZ_CP010848.1); D) R. toxicus WAC3373 (NZ_CP013292.1).
Fig 2.
R. toxicus groups with the Microbacteriaceae.
Maximum likelihood bootstrap phylogram of representative Actinobacteria showing strong support for placement of R. toxicus FH-79 in the Microbacteriaceae. Phylogeny based on concatenated 16S, gyrB, and secA1 sequences.
Fig 3.
Structure of the tunicamycin gene cluster (TGC) from R. toxicus and overview of tunicamycin biosynthetic pathway.
A) GC-content analysis of a 28-kb region surrounding the TGC. B) R. toxicus FH-79 TGC contains 12 genes with high homology to tun genes from S. chartreusis (tunA-L) and two additional genes (tunO and P) in two divergently transcribed operons. C) Hypothesized tunicamycin biosynthetic pathway. Incorporated fragments are highlighted in light blue. Adapted from [33, 34].
Fig 4.
Pigment and menaquinone biosynthetic clusters.
Gene clusters from R. toxicus FH-79 appearing to encode a carotenoid pigment (A) and menaquinone MK-10 (B). Scale bar ticks correspond to 1 kb.
Fig 5.
Maximum likelihood phylogenetic tree of putatively secreted serine proteases for R. toxicus FH-79 and C. michiganensis subsp. michiganensis NCPPB382.
Percentage of trees in which the associated taxa clustered together is shown next to the branches; values less than 70 have been omitted. R. toxicus FH-79 is designated with black diamonds; gene name and accession numbers are displayed in parentheses.
Fig 6.
Coding regions are depicted with black arrows and the non-coding CRISPR is in green. Scale bar ticks correspond to 5 kb.