Table 1.
Regulatory limits for ionophore coccidiostats in non-target feed following carry-over.
Table 2.
LC-MS/MS parameters for detection of ionophore coccidiostats in MRM mode.
Table 3.
Calibration curve points.
Fig 1.
Chromatogram of standard solution at the following concentrations: Nigericin at 25 ng ml-1, semduramicin sodium and lasalocid sodium at 3.125 ng ml-1, salinomycin sodium at 1.75 ng ml-1, monensin sodium at 3.125 ng ml-1, narasin at 1.75 ng ml-1 and maduramicin ammonium at 0.625 ng ml-1.
For each analyte, the most intense product ion was selected.
Fig 2.
Chromatogram of representative blank feed spiked with 1.25 mg kg-1 of nigericin.
For each analyte, the most intense product ion was selected.
Table 4.
Validation data.
Fig 3.
Chromatogram of representative blank feed spiked at the following concentrations: 1.25 mg kg-1 for nigericin, 0.125 mg kg-1 for semduramicin sodium, 0.625 mg kg-1 for lasalocid sodium, 0.350 mg kg-1 for salinomycin sodium, 0.625 mg kg-1 for monensin sodium, 0.350 mg kg-1 for narasin, 0.025 mg kg-1 for maduramicin ammonium.
Table 5.
Ionophore coccidiostat concentrations (mg kg-1 ± MU)* in feedstuffs at carry-over levels.