Fig 1.
Tissue types and glucosinolate variation in B. stricta.
(A) Tissue types in B. stricta. Cauline leaves and fruits are produced within one growing season, following bolting. (B) Aliphatic glucosinolates of B. stricta. Each glucosinolate type is derived from methionine or one of two branched-chain amino acids, valine or isoleucine. Two types are derived from valine.
Fig 2.
Effects of clipping treatments on fruit number.
Endogenous plants were randomly assigned to one of five treatments; for the removal treatments, 1/3rd of the total tissue in question was removed manually. Letters indicate significant pair-wise differences. The control, rosette, and cauline treatments are not significantly different, but flower and fruit removal significantly decreased fruit number. Error bars indicate standard error.
Table 1.
Effects of tissue removal treatments on fruit number.
Fig 3.
Effects of ambient herbivory on fruit number.
Individuals are plotted as points. The slope of the line of fit is equal to the selection gradient on damage to that tissue. Damage to fruits reduces fitness significantly more than loss of cauline leaf tissue (P<0.001).
Table 2.
Effects of ambient herbivory on fruit number.
Fig 4.
Variation in glucosinolate concentration between cauline leaves and fruits.
Fruits have a significantly higher concentration of glucosinolates than cauline leaves do (P<0.0001). Error bars indicate standard error.
Fig 5.
Variation in glucosinolate profile between cauline leaves and fruits.
A) The proportion of total glucosinolates that are branch chain-derived glucosinolates, B) The proportion of branch chain-derived that are valine-derived, and C) The proportion of valine-derived that are 2OH1ME. Tissues differ significantly for all three traits. Error bars indicate standard error.
Table 3.
Variation in glucosinolate traits.
Table 4.
Effects of glucosinolate traits on tissue damage.