Table 1.
Primers used in this study.
Fig 1.
Sequence alignment of all identified OBPs and CSPs in M. mediator.
A: Sequence alignment of OBPs, six conserved cysteine residues are marked in black; B: Sequence alignment of CSPs, four conserved cysteine residues are marked in black. Predicted signal peptides are boxed in the figure.
Fig 2.
Phylogenetic tree of OBPs and CSPs from Hymenoptera species.
Mmed (red): M. mediator; Ccun (purple): Chouioia cunea; Ssp (green): Sclerodermus sp.; Amel (blue): Apis mellifera.
Table 2.
OBPs and CSPs newly identified in antennae of M. mediator.
Fig 3.
qPCR analysis of MmedOBPs and MmedCSPs expression in different tissues.
The error bars represent standard error and the different small letters above each bar indicate significant differences in transcript abundances (P < 0.05).
Fig 4.
SDS-PAGE and western blot analysis of recombinant MmedCSP3.
M: Molecular weight marker; 1: Non-induced pET-30a (+) / MmedCSP3; 2: Induced pET-30a (+) / MmedCSP3; 3: pET-30a (+) / MmedCSP3 supernatant; 4: pET-30a (+) / MmedCSP3 pellet; 5: Purified MmedCSP3 with His-tag; 6: Purified MmedCSP3 without His-tag; 7: Western blot analysis of MmedCSP3.
Fig 5.
Immunocytochemical localization of MmedCSP3 in sensilla on female antennae.
(A): The sensilla basiconica type 2 was labeled strongly, whereas the sensilla placodea were not labeled. (B): The sensillum lymph in the sensilla basiconica type 2 was strong labeled. sp, s. placodea; st, s. trichodea; c, cavity; d, dendrites; p, pore; w, sensillum wall.
Fig 6.
Binding characteristic of selected ligands to MmedCSP3.
(A) Binding curve and relative Scatchard plot of bis-ANS to MmedCSP3. (B) Competitive binding curves of four selected plant volatiles to MmedCSP3 (C) Competitive binding curves of three sex pheromone components to MmedCSP3. (D) Competitive binding curved of two fatty acids to MmedCSP3.
Table 3.
Binding affinities of selected ligands with recombinant MmedCSP3.