Fig 1.
Development of five new molecular markers in Glu-A1 region.
(A) Organization of homologous Glu-A1 regions in the common wheat variety Renan, durum wheat Langdon and T. urartu G1812. The diagrams illustrating the organization of the various transposon elements and genes in the three Glu-A1 regions were adapted from previously published works [11–13]. Only the structures relevant to this work are shown. The letters ‘‘a” and ‘‘b” denote distinct copies of the duplicated genes. Positions of the five newly developed DNA markers (Xid3, Xid4, Xrj5, Xrj6 and Xrj7) are indicated by arrows. (B) Validation of the chromosomal specificity of the five newly developed Glu-A1 markers. The names and genome constitution of the materials were indicated on the top of the graph. The names of five markers were indicated on the right of corresponding photo, the corresponding length of PCR amplification product was indicated on the left.
Table 1.
Glu-A1 locus haplotypes detected in T. urartu, T. turgidum and common wheat populations.
Fig 2.
SDS-PAGE detection of HMW-GS composition in T. urartu Glu-A1 haplotypes.
The names of materials detected were marked on the top of the photo. The protein bands represented the T. urartu 1Ax or 1Ay subunits were marked on the right. The corresponding haplotypes were marked on the bottom. The arrow indicated the 1Ay-428339 that displayed the slowest electrophoretic mobility compared with other T. urartu 1Ay subunits.
Fig 3.
SDS-PAGE detection of HMW-GS composition in T. turgidum Glu-A1 haplotypes.
The names of materials were marked on the top of the photo. The protein bands represented the 1Ax subunits were indicated by arrows, and the protein bands represented the 1Ay subunits were indicated by arrowheads. The corresponding haplotypes were marked on the bottom.
Fig 4.
Phylogenetic network analysis of 11 Glu-A1 haplotypes.
The haplotypes detected in T. urartu were surrounded with black box, those in T. turgidum were surrounded with red box, and those in common wheat were surrounded with blue circle.