Table 1.
List of all primers for 12 markers used in genetic mapping of ClLL1.
Fig 1.
Phenotypes of leaf shapes in watermelon.
(a) Lobed and non-lobed leaf watermelon seedlings. (b) Two different leaf shapes in watermelon. Small symmetrical marginal protrusions are marked with red arrows. (c) Comparison of the first ten true leaves from lobed and non-lobed phenotype seedlings.
Table 2.
Detailed information of RNA-seq data of lobed and non-lobed leaf bulks.
Table 3.
Distribution of reliable SNPs and indels on watermelon chromosomes.
Fig 2.
Genetic mapping of the lobed leaf trait gene ClLL1 in watermelon.
(a) Primary mapping of ClLL1 using 93 F3:4 individuals. Gene ClLL1 was delimited to the region between markers W03041 and W01214. The numbers in brackets after the marker name indicate the numbers of recombinants. (b) Fine mapping of ClLL1. The gene ClLL1 was fine mapped in a l27.6-kb region between markers W08314 and W07061. (c) Marker genotypes of the recombinants near the lobed leaf gene ClLL1 between W08314 and W07061. The alleles are abbreviated according to their origin: A: Lobed leaf; B: Non-lobed leaf; H: Heterozygous.
Table 4.
Predicted genes between markers W08314 and W07061.
Fig 3.
Prediction and relative expression level of candidate genes in the ClLL1 region.
(a) 23 putative ORFs were predicted in a 127.6-kb region between makers W08314 and W07061. (b) The relative expression level of candidate genes in both lobed and non-lobed leaf plants. The data are presented as average values of three replicates (mean value ± SD). “*, **” represent significant differences at p < 0.05 and p < 0.01, respectively, according to the Student’s t-test. Actin was used as an internal control.
Fig 4.
Nucleotide polymorphisms of ORF18 and ORF22 among three watermelon genomes.
No nucleotide polymorphisms were identified in the cDNA sequences of ORF18 among three genomes. Two deletions (27-bp and 24-bp) were found in the second exon of ORF22 among three genomes. The homeodomain (HD) domain and leucine zipper (LZ) motif of ORF22 were predicted by the software Pfam (http://pfam.xfam.org/).