Fig 1.
Wide-field imaging system for an awake head-restrained mouse.
(A) Simplified schematic of the endoscope imaging system. The blue line indicates the illumination pathway and the green line indicates the light collection pathway. Illumination light from a blue LED was collected with a condenser lens, passes through an excitation filter, reflects off a dichroic mirror, and irradiated through an objective lens. The fluorescence image was focused on a CMOS camera. (B) Image of the endoscope system. (C) Schematic of experimental setup showing the chronic window implant above the dentate gyrus. (D) Point spread function of the imaging setup with (lower) and without (upper) the cannula. Lateral (center) and axial (right) intensity line profiles across a 0.5 μm fluorescent bead (black dots) were shown. The read lines indicate Gaussian fits.
Fig 2.
(A) View from start point of the virtual linear track. (B) Top view of the track. Scale bar, 10 cm.
Fig 3.
Imaging the dentate gyrus in behaving mice.
(A) Averaged fluorescence images across entire recording session. Scale bar, 100 μm. (B) Contours of putative cell bodies identified with a PCA and ICA-based cell sorting algorithm (Cellsort 1.0, Mukamel et al., 2009). Scale bar, 100 μm. (C) Traces of dF/F calcium signal were shown. Identified calcium transients are shown as red dots. The position of the mouse along the virtual track is shown at the bottom. Animal position and speed in the virtual track was shown in the bottom.