Fig 1.
Example of one of the analyzed pyramidal neurons.
(a) Confocal microscopy image of an intracellularly injected layer III pyramidal neuron of the human temporal cortex (Neuron 1 in Tables 1 and 2), visualized in 3D from high-resolution confocal stacks of images. (b) 3D reconstruction of the complete morphology of the cell shown in (a). (c) 3D reconstruction of the same neuron showing the apical dendrite in red and the four reconstructed basal dendrites in blue, green, orange and purple. We use the blue basal tree in (c) throughout the manuscript to illustrate the analysis performed. Scale bar (in (b)): 50 μm.
Fig 2.
Example of basal dendritic segment.
(a) High magnification confocal microscopy image showing a basal dendritic segment from Neuron 1. (b, c) Reconstruction of the dendritic shaft and spines shown in (a) in a solid (b) and mesh (c) view.
Fig 3.
First basal arborization of Neuron 1 illustrating the analysis (some of its characteristics are shown in Table 2).
(a) 3D representation of the basal network. The tree root is shown in black. (b) Zoom of a small part of the same dendrite (end of the dendritic segment shown in Fig 2) to illustrate the computation of the dendrite axis (i.e., the network in dark blue) and the attachment points of the spines (network events in red) from the reconstruction provided in the .vrml file (light blue).
Table 1.
Description of the analyzed apical dendrites.
The table shows the number of spines n, total length of the network |L| (in μm) average number of points per unit length in the network n/|L|, circumradius R (in μm), and number of branching points in the dendrite #BP.
Table 2.
Description of the analyzed basal dendrites grouped by neuron.
The table shows the number of spines n, total length of the network |L| (in μm), average number of points per unit length in the network n/|L|, circumradius R (in μm), and number of branching points in the dendrite #BP.
Fig 4.
Estimate of the intensity of the first basal arborization of Neuron 1 as a function of the distance (in μm) to the tree root.
Fig 5.
5% critical envelopes of the first basal arborization of Neuron 1.
(a) Estimation of 3D geometrically corrected inhomogeneous KLI function. (b) Estimation of 2D geometrically corrected inhomogeneous KLI function. (c) Estimation of 3D K function ignoring the network (the envelope is just below the red dotted line).
Fig 6.
Estimated 3D KLI functions used in the studentized permutation test.
(a) Estimated 3D KLI functions of all basal networks grouped by neuron in the distance range [0, 134.70] (g = 5 groups, p-value = 0.808). (b) Estimated 3D KLI functions of all apical networks forming a group and all basal networks forming another group in the distance range [0, 134.70] (g = 2, p-value = 0.109) (c) Estimated 3D KLI functions of all apical networks forming a group and the basal dendrites of Neurons 1, 3, 4 and 5 forming another group in the distance range [0, 165.96] (g = 2, p-value = 0.045).