Table 1.
Primers used for quantitative PCR.
Fig 1.
Effects of H2S and ethylene treatment on postharvest ripening (A) and chromatism values of L*, a*, b* of bananas (B).
Banana fingers were respectively fumigated with water, 1.0 mM solution of H2S donor NaHS, 1.0 g·L–1 ethylene donor ethephon solution or 1.0 mM aqueous solution of NaHS plus 1.0 g•L–1 ethephon solution for 0–6 d at 25°C as shown in lower right part of A. Photographs (A) were taken from Day 0 to Day 6, and chromatism values of L*, a* and b* (B) of banana were recorded on 0, 1, 3 and 5 d. The symbols * and ** in this figure stand for significant difference between ETH and ETH+H2S at P<0.05 and P<0.01, respectively. CK: control group; H2S: H2S treatment; ETH: ethylene treatment; ETH+H2S: ethylene plus H2S treatment.
Fig 2.
Effects of H2S and ethylene treatment on the firmness of banana of peel (A) and pulp (B) and on the activities of polygalacturonase (PG) in banana peel (C) and pulp (D).
Bananas were respectively fumigated with water, 1.0 mM aqueous solution of NaHS, 1.0 g·L–1 ethephon solution or 1.0 mM aqueous solution of NaHS plus 1.0 g·L–1 ethephon solution for 0–5 d at 25°C. Data were recorded on 0, 1, 3 and 5 d and expressed as means ± SD of three independent experiments with three replicates. The symbols * and ** in this figure stand for significant difference between ETH and ETH+H2S at P<0.05 and P<0.01, respectively. FW: fresh weight. CK: control group; H2S: H2S treatment; ETH: ethylene treatment; ETH+H2S: ethylene plus H2S treatment.
Fig 3.
Effects of H2S and ethylene treatment on the contents of total chlorophyll (A), chlorophyll a (B), chlorophyll b (C), carotenoid (D), total phenolics (E), flavonoid (F), soluble protein (G) in banana peel and reducing sugar (H) in banana pulp.
Bananas were respectively fumigated with water, 1.0 mM aqueous solution of NaHS, 1.0 g·L–1 ethephon solution or 1.0 mM aqueous solution of NaHS plus 1.0 g·L–1 ethephon solution for 0–5 d at 25°C. Data were recorded on 0, 1, 3 and 5 d and expressed as means ± SD of three independent experiments with three replicates. The symbols * and ** in this figure stand for significant difference between ETH and ETH+H2S at P<0.05 and P<0.01, respectively. FW: fresh weight. CK: control group; H2S: H2S treatment; ETH: ethylene treatment; ETH+H2S: ethylene plus H2S treatment.
Fig 4.
Effects of H2S and ethylene treatment on the production rate of superoxide anion (·O2−) (A), and contents of hydrogen peroxide (H2O2) (B), and malondialdehyde (MDA) (C) and ABTS radical scavenging activities (D) in banana peel.
Bananas were respectively fumigated with water, 1.0 mM aqueous solution of NaHS, 1.0 g·L–1 ethephon solution or 1.0 mM aqueous solution of NaHS plus 1.0 g·L–1 ethephon solution for 0–5 d at 25°C. Data were recorded on 0, 1, 3 and 5 d and expressed as means ± SD of three independent experiments with three replicates. The symbols * and ** in this figure stand for significant difference between ETH and ETH+H2S at P<0.05 and P<0.01, respectively. FW: fresh weight. CK: control group; H2S: H2S treatment; ETH: ethylene treatment; ETH+H2S: ethylene plus H2S treatment.
Fig 5.
Effects of H2S and ethylene treatment on the relative gene expression of MaACS1 (A), MaACS2 (B), MaACO1 (C), MaACO2 (D), MaETR (E), MaERS1 (F), MaERS2 (G) and MaPL (pectin lyase gene) (H) in banana peel.
Peels of bananas fumigated with 1.0 g·L–1 ethephon solution or 1.0 mM aqueous solutions of NaHS plus 1.0 g·L–1 ethephon solution were sampled on Days 0, 1 and 3 at 25°C. The symbols * and ** in this figure stand for significant difference between E and E+S at P<0.05 and P<0.01, respectively. Data were expressed as means ± SD of three independent experiments with three replicates. E: ethylene treatment; E+S: ethylene and H2S treatment.