Fig 1.
Location and sequence of the SLC6A4 promoter region targeted by DNA methylation analyses.
Numbers indicate nucleotide positions according to NCBI reference sequence NG_011747.2 (GeneBank). Underlined sequences correspond to primers used in PCR. CpG sites found to be methylated in the placental SLC6A4 gene are shown in bold.
Table 1.
Characteristics of newborns and their mothers stratified according to mother's glucose tolerance status.
Fig 2.
Placental SLC6A4 methylation levels correlate with maternal fasting glucose concentrations and placental SLC6A4 mRNA levels.
Scatterplots depict correlation of placental SLC6A4 methylation with (A) maternal fasting glucose levels in the 24th to 28th week of pregnancy, and (B) placental SLC6A4 mRNA levels. n, number of subjects; rp, Pearson's correlation coefficient; rs, Spearman's correlation coefficient; RER, relative expression ratio.
Table 2.
Association of placental SLC6A4 methylation with categorical variables of the study population.
Fig 3.
Placental SLC6A4 mRNA levels according to maternal glucose tolerance status and 5-HTTLPR/rs25531 genotype.
Shown are means and standard errors. p-values were 0.007, 0.316, and 0.176 for the main effect of diagnosis, main effect of genotype, and genotype x diagnosis interaction, respectively (two-way ANOVA). GDM, gestational diabetes mellitus; NGT, normal glucose tolerance; RER; relative expression ratio.