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Fig 1.

Light microscopy images of Nostoc sp. (A), Phormidium sp. (B) and Scytonema arcangeli (C) and their inoculum in plastic containers.

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Fig 2.

Wind tunnel test in the field.

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Fig 3.

Rapidly formed cyanobacterial crust images in the field at 4 and 12 months after inoculation.

(Con) control soil; (CY) cyanobacteria treated soil; (CST) cyanobacteria with SAP and TKS7 treated soil; (N-BSC) natural cyanobacterial crust. The photos of the 4 month old crusts were taken when the soil was wet. Photos of the 12 month old crusts and the N-BSC were taken when the soil was dry.

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Fig 4.

Fragment size distribution (A, B) and mean weight diameter values (C) of cyanobacterial crusts after 4 and 12 months of development. (Con) control soil; (CY) cyanobacteria treated soil; (CST) cyanobacteria with SAP and TKS7 treated soil; (N-BSC) natural cyanobacterial crust. Values represent the means of quadruplicate measurements. Values with the same letter are not significantly different according to the multiple comparison test (95% Duncan’s post hoc tests). Error bars represent the standard errors.

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Table 1.

Biological and physical measurements of cyanobacterial crusts after 4 and 12 months of development.

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Fig 5.

Threshold friction velocity values of cyanobacterial crusts after 12 months of development.

The images were taken after the wind tunnel test. (Con) control soil; (CY) cyanobacteria treated soil; (CST) cyanobacteria with SAP and TKS7 treated soil; (N-BSC) natural cyanobacterial crust. Values represent the means of triplicate measurements. Values with the same letter are not significantly different according to the multiple comparison test (95% Duncan’s post hoc tests). Error bars represent the standard errors.

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Fig 6.

Total carbohydrate (A), chlorophyll a (B), organic carbon (C), soil respiration (D), carbon fixation (E) and microbial biomass carbon (F) of cyanobacterial crusts after 4 and 12 months of development. (Con) control soil; (CY) cyanobacteria treated soil; (CST) cyanobacteria with SAP and TKS7 treated soil; (N-BSC) natural cyanobacterial crust. Values represent the means of triplicate measurements. Values with the same letter are not significantly different according to the multiple comparison test (95% Duncan’s post hoc tests). Error bars represent the standard errors.

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Fig 7.

Relationship between MWD values and each soil content factor (total carbohydrate, chlorophyll a, organic carbon).

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Fig 8.

Quantum yield efficacy of PSII (ΔF/Fmʹ) (A, B) and relative electron transport rate (rETR) (C, D) of cyanobacterial crust after 4 and 12 months of development. (Con) control soil; (CY) cyanobacteria treated soil; (CST) cyanobacteria with SAP and TKS7 treated soil; (N-BSC) natural cyanobacterial crust. Values represent the means of triplicate measurements. Error bars represent the standard errors.

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Fig 9.

Soil surface of the inoculated crusts immediately after heavy rain in the field.

(Con) control soil; (CY) cyanobacteria treated soil; (CST) cyanobacteria with SAP and TKS7 treated soil.

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