Fig 1.
Expression levels of HMGB3 mRNA and protein are increased in CRC tissues.
(A) qRT-PCR analysis of HMGB3 expression in 34 paired CRC tissues and adjacent normal tissues. HMGB3 expression level was normalized to GAPDH and the results were presented as the fold-change in tumor tissues relative to the matched adjacent normal tissues. Error bars indicate mean ± standard deviation of 3 independent experiments. (B) The value of ΔCt was used to show the expression level of HMGB3 (ΔCt = CtHMGB3 –CtGAPDH) in the 34 paired human CRC tissues (T) and adjacent normal tissues (N) (p < 0.05). The differences between tumor group and normal group were tested by using a Paired-samples t-test. (C) Western blot analysis of HMGB3 protein expression in seven pair CRC tissues compared with the normal tissues. The differences between tumor group and normal group were tested by using a Independent-Samples t-test. (D) Representative HMGB3 staining in CRC specimens. Left, normal tissue, Scale bars = 50 μm. Middle, CRC tissues, Scale bars = 50μm. Right, CRC tissues, Scale bars = 20 μm. The HMGB3 index was calculated as that the number of HMGB3 positive cells divided by the number of total cells ×100% (magnification, ×200). The differences between tumor group and normal group were tested by using a Independent-Samples t-test. Error bars represent the mean ± SD of 5 different fields. ***, p < 0.001.
Table 1.
Clinicopathologic characteristics of HMGB3 expression in CRC patients.
Fig 2.
HMGB3 promote CRC cells proliferation and migration in vitro.
(A) qRT-PCR analysis of HMGB3 expression in six CRC cell lines. The differences between independent experimental groups were tested by using Independent-Samples t-test. Error bars indicate mean ± SD of 3 independent experiments. ***, p < 0.001. (B) qRT-PCR and western blot analyses of HMGB3 mRNA and protein levels after the transfection of the sh-HMGB3 or HMGB3 plasmid. The differences between independent experimental groups were tested by using Independent-Samples t-test. ***, p < 0.001. (C) Effects of sh-HMGB3 and oe-HMGB3 on cell proliferation were determined by CCK8 cell proliferation assay. Error bars represent the mean ± SD of 5 independent experiments. The differences between independent experimental groups were tested by using Independent-Samples t-test. ***, p < 0.001. (D) The proliferation ability was determined by colony formation assay of the cell SW620 and HT29 after inhibiting the expression of HMGB3. The bar chart represents the colony number. Error bars represent the mean ± SD of 3 independent experiments. The differences between independent experimental groups were tested by using Independent-Samples t-test. **, p < 0.01; ***, p < 0.001. (E) Cell cycle G1 arrest after knocking down HMGB3 measured by flow cytometry. (F) The migration capacity was determind by transwell assays. The bar chart represents the migration cell numbers. Error bars represent the mean ± SD of 5 different field. The differences between independent experimental groups were tested by using Independent-Samples t-test. ***, p < 0.001. (G) Effects of sh-HMGB3 or oe-HMGB3 on cell migration ability were determined by Wound healing assay.
Fig 3.
HMGB3 promotes CRC cell growth and migration through WNT/β-catenin pathway.
Western blot was performed to detect HMGB3, β-catenin, c-Myc, and MMP7 expression after hmgb3 knocking down (A) or up-regulating (B). Data are mean ± SD for triplicate samples. GAPDH served as the loading control. The differences between independent experimental groups were tested by using Independent-Samples t-test. (C) Cells were transfected with TOP-Flash or control FOP-Flash reporter to determine reporter activities 48h later. The values of TOP-Flash and FOP-Flash were normalized to the value of pRL-SV40, the bar chart represented the ratios of TOP/FOP. Values are mean ± SD for triplicate samples. The differences between independent experimental groups were tested by using Independent-Samples t-test. *, p < 0.05; **, p < 0.01. (D) The expression of c-Myc, MMP7 and β-actin in SW480 and HCT116 cells were detected by western blot after increasing the expression of HMGB3 or increasing the expression of HMGB3 but inhibited β-catenin expression. β-actin served as the loading control. Error bars represent the mean ± SD of 3 independent experiments. The differences between independent experimental groups were tested by using Independent-Samples t-test. **, p < 0.01; ***, p < 0.001.