Table 1.
Sequences of capture probe (1) and miR-122 target (2).
Fig 1.
Schematic of the assay for miRNA based on combining dynamic labeling of specific single bases and detection using single molecule arrays.
Fig 2.
Dynamic chemistry labeling of biotin into an immobilized abasic PNA probe on a superparamagnetic bead.
Fig 3.
Plot of AEB determined using the assay in Fig 1 against concentration of calibrators for miR-122 (circles) and the same molecule with a single base mismatch at the 9th position (squares) spiked into buffer.
The sequences of these two molecules are shown in S2 Table. Error bars (±1 s.d.) based on duplicate measurements are smaller than the size of the data point.
Fig 4.
(A) Scatter plot of AEB for miR-122 measured in the serum of 4 healthy volunteers and 4 individuals after overdosing on acetaminophen. The dotted lines represent the mean assay (buffer) background ± 1 s.d.. (B) Correlation of concentration of miR-122 in the serum of patients determined using Simoa and PCR (r2 = 0.93; slope = 0.64).