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Fig 1.

Flowchart of participants through the study.

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Table 1.

Serum levels of calcium, vitamin D, Phosphorus (P), PTH and FGF-23.

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Table 2.

Expression of TLR7, TLR9, IL-6, IFN-γ, VDR, CYP27b1 and CYP24a1 in B and T lymphocytes.

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Fig 2.

Effect of 25 and 1,25 vitamin D on TLR7 and TLR9 expression (MFI) in B lymphocytes (Figs A and B) and T lymphocytes (Figs C and D) in presence of healthy or uremic serum (US) and after CYP24 silencing (siRNA).

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Fig 3.

Effect of 25 and 1,25 vitamin D on IL-6 (MIF) and IFN-γ expression (MFI) in B lymphocytes (Figs A and B) and T lymphocytes (Figs C and D) in presence of healthy or uremic serum (US) and after CYP24 silencing (siRNA).

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Fig 4.

Effect of 25 and 1,25 vitamin D on VDR expression in B and T lymphocytes (Figs A and C) in the presence of healthy or uremic serum (US) and after CYP24 silencing (siRNA). CYP24 expression on healthy serum (HS); healthy serum+FGF-23 (HS+FGF-23) and uremic serum+FGF-23 incubation (US+FGF-23) (MFI) in B lymphocytes (Fig B) and T lymphocytes (Fig D). E: Effect of 25 and 1,25 vitamin D on NF-κB activation in PBMC in the presence of healthy or uremic serum (US) and after CYP24 silencing (siRNA).

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