Fig 1.
Process of eDNA capture involves concentration, extraction, inhibitor removal, and PCR amplification of a water sample.
Fig 2.
Schematic for the study design of our eDNA capture optimization experiment.
Table 1.
Model ranking of occupancy models evaluating the probability of detecting eDNA from turbid water using various DNA extraction techniques (“extraction”) and the effect of water collection date.
Table 2.
The probability of detection of eDNA estimated based on the best model (Table 1) of collection date and extraction method.
Table 3.
Model ranking of occupancy models evaluating the probability of detecting eDNA from turbid water using DNA concentration, extraction technique, and inhibitor removal (“IRT”).
Table 4.
The probability of detection estimated based on the best model: P(concentration+extraction+IRT).
Table 5.
Model ranking of occupancy models evaluating the probability of detecting eDNA from turbid water with two amplification methods (cPCR and qPCR).
Fig 3.
Sensitivity, or the ability to detect low levels of eDNA, with cPCR and qPCR amplification methods across dilutions of eDNA samples.