Table 1.
Malassezia pachydermatis studied, including original animal host, pathology, and LSU rRNA, ITS rRNA, CHS2 and beta-tubulin genotypes.
Table 2.
Main differential phenotypic characteristics of the studied M. pachydermatis strains.
Fig 1.
Growth patterns of M. pachydermatis strains in the Tween diffusion test with SGA and YNBA, after 7 days of incubation at 32°C.
The growth patterns were defined as good growth (+); weak growth (w); growth at a distance of the well where the substrate was placed (+a); ring of growth inhibition at a distance of the well (+b); growth inhibition (-); *: lipid-dependent strain.
Fig 2.
Growth of the neotype strain of M. pachydermatis (CBS 1879) on YNBA supplemented with peptone (10 g/1000 ml) and on YNBA supplemented with glucose (40 g/1000 ml) and peptone (10 g/1000 ml).
None of the lipid-dependent strains (MA-366, MA-374 and MA-380) was able to grow on this media.
Fig 3.
Molecular phylogenetic tree inferred from maximum likelihood analysis of LSU sequences of Malassezia pachydermatis strains.
Bootstrap values > 70% in 1,000 replications are shown at the nodes. Sequences of M. furfur CBS 1878 and CBS 7019, Ustilago maydis ATCC MYA-4924 and Cryptococcus neoformans CBS 132 as outgroup were selected for the tree construction.* Lipid-dependent strains.